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Archives of Microbiology

, Volume 199, Issue 2, pp 247–257 | Cite as

A comparative study of the evolution of cellobiose utilization in Escherichia coli and Shigella sonnei

  • Asha Mary Joseph
  • Robert Sonowal
  • Subramony MahadevanEmail author
Original Paper
  • 258 Downloads

Abstract

The chb operon of Escherichia coli is involved in the utilization of chitooligosaccharides. While acquisition of two classes of mutations leading to altered regulation of the chb operon is necessary to confer the ability to utilize the glucose disaccharide cellobiose to wild-type strains of E. coli, in the closely related organism Shigella sonnei, Cel+ mutants arise relatively faster, requiring only a single mutational event. In Type I mutants, the insertion of IS600 at −21 leads to ChbR regulator-independent, constitutive expression of the operon. In Type II mutants, the insertion of IS2/600 within the distal binding site of the negative regulator NagC leads to ChbR-dependent cellobiose-inducible expression of the operon. These studies underscore the significance of strain background, specifically the diversity of transposable elements, in the evolution of novel metabolic functions. Constitutive expression of the chb operon also enables utilization of the aromatic β-glucosides arbutin and salicin, implying that the chb structural genes are inherently promiscuous.

Keywords

chb operon Cellobiose Shigella sonnei IS elements 

Notes

Acknowledgments

This work was supported by an institutional grant from the Department of Biotechnology (DBT), Govt. of India, to the Indian Institute of Science and infrastructural support from the Department of Science and Technology (DST) under the FIST Program and the Universities Grants Commission (UGC), Govt. of India. AMJ thanks the Council for Scientific and Industrial Research (CSIR), Govt. of India, for a senior research fellowship.

Supplementary material

203_2016_1299_MOESM1_ESM.pdf (331 kb)
Supplementary material 1 (PDF 330 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Asha Mary Joseph
    • 1
  • Robert Sonowal
    • 1
    • 2
  • Subramony Mahadevan
    • 1
    Email author
  1. 1.Department of Molecular Reproduction, Development and GeneticsIndian Institute of ScienceBangaloreIndia
  2. 2.Department of PathologyEmory University School of MedicineAtlantaUSA

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