Three yeast strains designated as S44, XF1 and XF2, respectively, were isolated from Scolytus scheryrewi Semenov of apricot tree in Shule County, Xinjiang, China, and were demonstrated to be a new member of the genus Candida by sequence comparisons of 26S rRNA gene D1/D2 domain and internal transcribed spacer (ITS) region. BLASTn alignments on NCBI showed that the similarity of 26S rRNA gene sequences of S44 (type strain) to all sequences of other Candida yeasts was very low (≦93 %). The phylogenetic tree based on the 26S rRNA gene D1/D2 domain and ITS region sequences revealed that the strain S44 is closely related to C. blattae, C. dosseyi, C. pruni, C. asparagi, C. fructus and C. musae. However, the strain S44 is distinguished from these Candida species by the physiological characteristics. Moreover, the strain S44 formed typical pseudohyphae when grown on cornmeal agar at 25 °C for 7 days, but did not form ascospores in sporulation medium for 3–4 weeks. Therefore, the name Candida xinjiangensis is proposed for the novel species, with S44 (=KCTCT27747) as the type strain.
Apricot tree Candida xinjiangensis sp. nov. China Scolytus scheryrewi
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The authors give thanks to Dr. Jianping-Wang for reviewing the manuscript. The work was supported by the Xinjiang Uygur Autonomous Region Nonprofit Research Institutes’ fundamental research Project fund (No. KY2015062) and the grant of the Beijing Municipal Science and technology Project (No. Z141100002614006).
Supplementary Fig. 1Phylogenetic relationships between Candida xinjiangensis sp. nov. S44T and members of the Candida clade inferred using neighbor-joining analysis (Kimura two-parameter mode) of combined sequences of the D1/D2 domains of the 26S rRNA gene. Kodamaea anthophila represents the outgroup. Scale represents 0.02 nucleotide substitutions per site. Bootstrap values (%) based on 1000 replications are given on each node. Bar means 1 % sequence divergence. Reference sequences were retrieved from GenBank or CBS under the accession numbers indicated in parentheses. (TIFF 1566 kb)
Supplementary Fig. 2Phylogenetic relationships between Candida xinjiangensis sp. nov. S44T and members of the Candida clade inferred using neighbor-joining analysis (Kimura two-parameter mode) of combined sequences of the ITS regions. Kodamaea anthophila represents the outgroup. Scale represents 0.02 nucleotide substitutions per site. Bootstrap values (%) based on 1000 replications are given on each node. Bar means 1 % sequence divergence. Reference sequences were retrieved from GenBank or CBS under the accession numbers indicated in parentheses. (TIFF 1342 kb)
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