A Gram-stain-negative, strictly aerobic, orange-colored, rod-shaped, chemoheterotrophic bacterium, designated HG732T, was isolated from marine sediment in Japan. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel marine strain was affiliated with the family Flavobacteriaceae of the phylum Bacteroidetes and that it shared the highest (94.1 %) sequence similarity with Kriegella aquimaris KMM 3665T. The strain could be differentiated phenotypically from related members of the family Flavobacteriaceae. Major fatty acids of strain HG732T were iso-C15:1 G, iso-C15:0 and iso-C17:0 3-OH. The polar lipid profile consisted of phosphatidylglycerol, three unidentidied aminolipids and two unidentified lipids. The DNA G+C content of the strain was determined to be 35.2 mol%, and the major respiratory quinone was identified as menaquinone 6 (MK-6). From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel genus in the family Flavobacteriaceae, for which the name Aurantibacter crassamenti gen. nov., sp. nov. is proposed. The type strain of A. crassamenti gen. nov., sp. nov. is HG732T (= KCTC 52207T = NBRC 112211T).
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Abell GC, Bowman JP (2005) Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean. FEMS Microbiol Ecol 51:265–277
Barbeyron T, L’Haridon S, Corre E, Kloareg B, Potin P (2001) Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [Cytophaga] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov. Int J Syst Evol Microbiol 51:985–997
Bernardet JF, Bowman JP (2006) The genus Flavobacterium. In: Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E (eds) The prokaryotes: a handbook on the biology of bacteria, vol 7, 3rd edn. Springer, New York, pp 481–531
Bernardet JF, Segers P, Vancanneyt M, Berthe F, Kersters K, Vandamme P (1996) Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148
Bernardet JF, Nakagawa Y, Holmes B (2002) Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070
Collins MD, Jones D (1981) A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin-layer chromatography and high performance liquid chromatography. J Appl Bacteriol 51:129–134
Collins CH, Lyne PM (1984) Microbiological methods, 5th edn. Butterworth, London
Cottrell MT, Kirchman DL (2000) Natural assemblages of marine proteobacteria and members of the Cytophaga–Flavobacter cluster consuming low- and high-molecular-weight dissolved organic matter. Appl Environ Microbiol 66:1692–1697
DeLong EF, Franks DG, Alldredge AL (1993) Phylogenetic diversity of aggregate-attached versus free-living marine bacterial assemblages. Limnol Oceanogr 38:924–934
Dittmer JC, Lester RL (1964) A simple, specific spray for the detection of phospholipids on thin-layer chromoatograms. J Lipid Res 15:126–127
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
Garrity GM, Holt JG (2001) The road map to the Manual. In: Boone DR, Castenholz RW, Garrity GM (eds) Bergey’s manual of systematic bacteriology, vol 1, 2nd edn. Springer, New York, pp 119–166
Glöckner FO, Fuchs BM, Amann R (1999) Bacterioplankton composition of lakes and oceans: a first comparison based on fluorescence in situ hybridization. Appl Environ Microbiol 65:3721–3726
Hansen GH, Sørheim R (1991) Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods 13:231–241
Hu J, Yang QQ, Ren Y, Zhang WW, Zheng G, Sun C, Pan J, Zhu XF, Zhang XQ, Wu M (2015) Maribacter thermophilus sp. nov., isolated from an algal bloom in an intertidal zone, and emended description of the genus Maribacter. Int J Syst Evol Microbiol 65:36–41
Ivanova EP, Nedashkovskaya OI, Chun J, Lysenko AM, Frolova GM, Svetashev VI, Vysotskii MV, Mikhailov VV, Huq A, Colwell RR (2001) Arenibacter gen. nov., new genus of the family Flavobacteriaceae and description of a new species, Arenibacter latericius sp. nov. Int J Syst Evol Microbiol 51:1987–1995
Jackson SA, Kennedy J, Morrissey JP, O’Gara F, Dobson AD (2015) Maribacter spongiicola sp. nov. and Maribacter vaceletii sp. nov., isolated from marine sponges, and emended description of the genus Maribacter. Int J Syst Evol Microbiol 65:2097–2103
Jeong SH, Jin HM, Kim JM, Jeon CO (2013) Arenibacter hampyeongensis sp. nov., a marine bacterium isolated from a tidal flat. Int J Syst Evol Microbiol 63:679–684
Jooste PJ (1985) The taxonomy and significance of Flavobacterium–Cytophaga strains from dairy sources. Ph.D. thesis, University of the Orange Free State, South Africa
Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721
Kimura M (1983) The neutral theory of molecular evolution. Cambridge University Press, Cambridge
Kirchman DL (2002) The ecology of Cytophaga–Flavobacteria in aquatic environments. FEMS Microbiol Ecol 39:91–100
Komagata K, Suzuki K (1987) Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
Ludwig W, Klenk HP (2001) Overview: a phylogenetic backbone and taxonomic framework for procaryotic systematics. In: Boone DR, Castenholz RW, Garrity GM (eds) Bergey’s manual of systematic bacteriology, vol 1, 2nd edn. Springer, New York, pp 49–66
Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218
Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167
Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinines and polar lipids. J Microbiol Methods 2:233–241
Nedashkovskaya OI, Kim SB, Han SK, Lysenko AM, Rohde M, Rhee MS, Frolova GM, Falsen E, Mikhailov VV, Bae KS (2004a) Maribacter gen. nov., a new member of the family Flavobacteriaceae, isolated from marine habitats, containing the species Maribacter sedimenticola sp. nov., Maribacter aquivivus sp. nov., Maribacter orientalis sp. nov. and Maribacter ulvicola sp. nov. Int J Syst Evol Microbiol 54:1017–1023
Nedashkovskaya OI, Suzuki M, Vancanneyt M, Cleenwerck I, Lysenko AM, Mikhailov VV, Swings J (2004b) Zobellia amurskyensis sp. nov., Zobellia laminariae sp. nov. and Zobellia russellii sp. nov., novel marine bacteria of the family Flavobacteriaceae. Int J Syst Evol Microbiol 54:1643–1648
Nedashkovskaya OI, Vancanneyt M, Cleenwerck I, Snauwaert C, Kim SB, Lysenko AM, Shevchenko LS, Lee KH, Park MS, Frolova GM, Mikhailov VV, Bae KS, Swings J (2006) Arenibacter palladensis sp. nov., a novel marine bacterium isolated from the green alga Ulva fenestrata, and emended description of the genus Arenibacter. Int J Syst Evol Microbiol 56:155–160
Nedashkovskaya OI, Suzuki M, Kim SB, Mikhailov VV (2008) Kriegella aquimaris gen. nov., sp. nov., isolated from marine environments. Int J Syst Evol Microbiol 58:2624–2628
Park S, Jung YT, Park JM, Won SM, Yoon JH (2015) Maribacter confluentis sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol 65:3079–3085
Perry LB (1973) Gliding motility in some non-spreading flexibacteria. J Appl Microbiol 36:227–232
Reichenbach H (1992) Flavobacteriaceae fam. nov. In validation of the publication of new names and new combinations previously effectively published outside the IJSB, list no. 41. Int J Syst Bacteriol 42:327–329
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. MIDI Inc, Newark
Sun F, Wang B, Du Y, Liu X, Lai Q, Li G, Luo J, Shao Z (2010) Arenibacter nanhaiticus sp. nov., isolated from marine sediment of the South China Sea. Int J Syst Evol Microbiol 60:78–83
Tamura K, Peterson D, Petersen N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using Maximum Likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882
Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
Worliczek HL, Kämpfer P, Rosengarten R, Tindall RBJ, Busse HJ (2007) Polar lipid and fatty acid profiles-re-vitalizing old approaches as a modern tool for the classification of mycoplasmas? Syst Appl Microbiol 30:355–370
Yoon J, Adachi K, Kasai H (2016) Spongiiferula fulva gen. nov., sp. nov., a bacterium of the family Flavobacteriaceae isolated from a marine sponge. Curr Microbiol 73:15–21
Zhang GI, Hwang CY, Kang SH, Cho BC (2009) Maribacter antarcticus sp. nov., a psychrophilic bacterium isolated from a culture of the Antarctic green alga Pyramimonas gelidicola. Int J Syst Evol Microbiol 59:1455–1459
This work was supported by the New Energy and Industrial Technology Development Organization (NEDO) and in part by a research grant (2009–2011) of the Institute for Fermentation, Osaka, Japan.
Conflict of interest
The authors declare that they have no conflict of interest.
Human participants and/or animals
This article does not contain any studies with human participants or animals performed by any of the authors.
An erratum to this article is available at http://dx.doi.org/10.1007/s00203-016-1302-9.
Communicated by Erko Stackebrandt.
About this article
Cite this article
Yoon, J., Kasai, H. Aurantibacter crassamenti gen. nov., sp. nov., a bacterium isolated from marine sediment. Arch Microbiol 199, 85–91 (2017). https://doi.org/10.1007/s00203-016-1280-y
- Aurantibacter crassamenti gen. nov., sp. nov.
- Marine sediment
- 16S rRNA gene
- Polyphasic taxonomy