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Proteomic analysis of Mortierella isabellina M6-22 during cold stress

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Abstract

We aimed to gain a better understanding of cold adaption in Mortierella isabellina M6-22 by using proteomics approaches. The temperature range and optimal temperature for M6-22 growth were investigated, and composition changes in fatty acids were analyzed. Accompanied with the 2-D gel electrophoresis, MALDI-TOF/TOF–MS analysis was conducted to characterize alterations in protein profiling in M6-22 cultured at 30 °C for 24 h and 15 °C for another 24 h when compared with those cultured at 30 °C for 48 h. Gene Ontology (GO) cluster analysis was finally conducted for successfully identified proteins. M6-22 cells could grow well at temperatures ranging from 15 to 30 °C. As temperature decreased from 30 to 15 °C, LA and GLA significantly increased from 11.63 to 17.85 % and from 9.12 to 13.19 %, respectively, while oleic acid significantly decreased from 47.25 to 36.53 %. Proteomics analyses revealed 111 differentially expressed protein spots, among which 5 unique proteins (A38, A40, A47, A49 and A58), 29 up-regulated proteins and 10 down-regulated proteins were identified by MALDI-TOF/TOF–MS. GO enrichment analysis demonstrated that these proteins mainly involved in glycolytic pathway (A34 and A50), electron transport (A28), ATP production (A35 and B39) and protein modification (A38). A total of 44 differentially expressed proteins have been successfully identified in M. isabellina M6-22 cultured at 15 °C. These proteins may play important roles in cold adaption via regulation of ATP synthesis, activation of cold-adaptive proteins, degradation of needless protein, accumulation of PUFAs, etc.

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Acknowledgments

This study was supported by a grant from National Natural Science Foundation of China (Nos. 31160016 and 31260034). (1) National Natural Science Foundation of China (31160016): “Effects of biosynthesis inhibition of polyunsaturated fatty acids on the cold adaptation of M. isabellina M6-22 to low temperature.” (2) National Natural Science Foundation of China (31260034): “Studies on the temporal regulation of viral transcription during the development of Sulfolobus virus STSV2.”

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Authors and Affiliations

  1. Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 South Jingming Road, Kunming, 650500, China

    Binbin Hu, Minzhou Luo, Xiuling Ji, Lianbing Lin, Yunlin Wei & Qi Zhang

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  1. Binbin Hu
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  2. Minzhou Luo
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  3. Xiuling Ji
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  4. Lianbing Lin
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  5. Yunlin Wei
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  6. Qi Zhang
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Correspondence to Qi Zhang.

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All authors declare that they have no conflict of interests to state.

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Communicated by Erko Stackebrandt.

Binbin Hu and Minzhou Luo should be regarded as co-first authors.

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Hu, B., Luo, M., Ji, X. et al. Proteomic analysis of Mortierella isabellina M6-22 during cold stress. Arch Microbiol 198, 869–876 (2016). https://doi.org/10.1007/s00203-016-1238-0

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  • DOI: https://doi.org/10.1007/s00203-016-1238-0

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