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Streptomyces zhihengii sp. nov., isolated from rhizospheric soil of Psammosilene tunicoides

Archives of Microbiology volume 198, pages 743–749 (2016)Cite this article

Abstract

An actinomycete strain, designated YIM T102T, was isolated from the rhizospheric soil of Psammosilene tunicoides W. C. Wu et C. Y. Wu collected from Lijiang, Yunnan Province, China. The taxonomic position of the new isolate was investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YIM T102T belongs to the genus Streptomyces. Strain YIM T102T was most closely related to Streptomyces eurocidicus NRRL B-1676T with a pairwise 16S rRNA gene sequence similarity of 98.9 %. However, DNA–DNA relatedness value between strain YIM T102T and S. eurocidicus NBRC 13491T was found to be 37.8 ± 1.8 %. The menaquinone composition detected for strain YIM T102T was MK-9 (H6) and MK-9 (H8), while the major fatty acids were summed feature 4 (38.0 %), anteiso-C15:0 (13.1 %), iso-C16:0 (10.1 %), summed feature 3 (9.8 %) and C16:0 (9.0 %) and iso-C15:0 (5.2 %). The whole-cell hydrolysates contained galactose, glucose, ribose and mannose, along with ll-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan. The DNA G+C content was 70.7 mol%. Strain YIM T102T also exhibited antagonistic activity against Alternaria alternata, Alternaria brassicae and Colletotrichum nicotianae Averna, based on the findings from the comparative analyses of phenotypic and genotypic characteristics; it is proposed that strain YIM T102 represents a novel species of the genus Streptomyces, for which the name Streptomyces zhihengii sp. nov. is proposed. The type strain is YIM T102T (=KCTC 39115T = DSM 42176T = CGMCC 4.7248T).

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Acknowledgments

The authors are grateful to Dr. Tomohiko Tamura (NBRC, Japan) for providing the reference type strain. This research was supported by Projects of Ministry of Science, ICT and Future Planning of Korean government (NRF-2013M3A9A5076601) and the Deanship of Scientific Research at King Saud University (Research Group No PRG-1436-27). W-J Li was also supported by Project Supported by Guangdong Province Higher Vocational Colleges and Schools Pearl River Scholar Funded Scheme (2014).

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Affiliations

  1. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, China

    Mei-Juan Huang & Wen-Jun Li

  2. College of Landscape Architecture, Southwest Forestry University, Kunming, 650224, China

    Mei-Juan Huang, Jing-Jing Fei & Hai-Quan Huang

  3. State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China

    Nimaichand Salam, Min Xiao & Wen-Jun Li

  4. Microbial Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea

    Chang-Jin Kim

  5. College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

    Wael N. Hozzein

  6. Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62511, Egypt

    Wael N. Hozzein

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  1. Mei-Juan Huang
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  2. Jing-Jing Fei
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  3. Nimaichand Salam
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Correspondence to Hai-Quan Huang or Wen-Jun Li.

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Communicated by Erko Stackebrandt.

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Huang, MJ., Fei, JJ., Salam, N. et al. Streptomyces zhihengii sp. nov., isolated from rhizospheric soil of Psammosilene tunicoides . Arch Microbiol 198, 743–749 (2016). https://doi.org/10.1007/s00203-016-1233-5

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Keywords

  • Streptomyces zhihengii sp. nov.
  • Rhizosphere soil
  • Lijiang
  • Psammosilene tunicoides