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Aeromicrobium halotolerans sp. nov., isolated from desert soil sample

Abstract

A Gram-positive, aerobic, and non-motile, rod-shaped actinomycete strain, designated YIM Y47T, was isolated from soils collected from Turpan desert, China, and subjected to a polyphasic taxonomic study. Phylogenetic analysis indicated that strain YIM Y47T belonged to the genus Aeromicrobium. YIM Y47T shared highest 16S rRNA gene sequence similarities with Aeromicrobium massiliense JC14T (96.47 %). Growth occurs at 20–45 °C (optimum at 30 °C), pH 6.0–8.0 (optimum at pH 7.0), and salinities of 0–7.0 % NaCl (optimum at 4.0 %). The strain YIM Y47T exhibits chemotaxonomic features with menaquinone-7 (MK-7) as the predominant quinone, C16:0, C18:1 ω9c and 10-methyl C18:0 (>10 %) as major fatty acids. The cell-wall peptidoglycan of strain YIM Y47T contained ll-diaminopimelic acid as the diagnostic diamino acid. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and unknown phospholipids. The G+C content of the genomic DNA of strain YIM Y47T was found to be 44.7 mol%. On the basis of phylogenetic analyses and phenotypic data, it is proposed that strain YIM Y47T should be classified as representing a novel species of the genus Aeromicrobium, with the name Aeromicrobium halotolerans sp. nov. The type strain is YIM Y47T (=KCTC 39113T=CGMCC 1.15063T=DSM 29939T=JCM 30627T).

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Acknowledgments

The authors are grateful to Prof. Dr. Hans Peter Klenk (DSMZ, Germany) for providing the reference type strains and Prof. Dr. Wen-Jun Li (Yunnan University, China) for experimental guidance. This research work was supported by Key Project of China Ministry of Science and Technology (2013BAD16B05).

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Correspondence to Chang-Tian Li or Tae-Hoo Yi.

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Communicated by Erko Stackebrandt.

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Yan, ZF., Lin, P., Chu, X. et al. Aeromicrobium halotolerans sp. nov., isolated from desert soil sample. Arch Microbiol 198, 423–427 (2016). https://doi.org/10.1007/s00203-016-1196-6

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  • DOI: https://doi.org/10.1007/s00203-016-1196-6

Keywords

  • Aeromicrobium halotolerans sp. nov.
  • Turpan desert
  • Polyphasic taxonomy