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Aeromicrobium halotolerans sp. nov., isolated from desert soil sample

Archives of Microbiology volume 198, pages 423–427 (2016)Cite this article

Abstract

A Gram-positive, aerobic, and non-motile, rod-shaped actinomycete strain, designated YIM Y47T, was isolated from soils collected from Turpan desert, China, and subjected to a polyphasic taxonomic study. Phylogenetic analysis indicated that strain YIM Y47T belonged to the genus Aeromicrobium. YIM Y47T shared highest 16S rRNA gene sequence similarities with Aeromicrobium massiliense JC14T (96.47 %). Growth occurs at 20–45 °C (optimum at 30 °C), pH 6.0–8.0 (optimum at pH 7.0), and salinities of 0–7.0 % NaCl (optimum at 4.0 %). The strain YIM Y47T exhibits chemotaxonomic features with menaquinone-7 (MK-7) as the predominant quinone, C16:0, C18:1 ω9c and 10-methyl C18:0 (>10 %) as major fatty acids. The cell-wall peptidoglycan of strain YIM Y47T contained ll-diaminopimelic acid as the diagnostic diamino acid. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and unknown phospholipids. The G+C content of the genomic DNA of strain YIM Y47T was found to be 44.7 mol%. On the basis of phylogenetic analyses and phenotypic data, it is proposed that strain YIM Y47T should be classified as representing a novel species of the genus Aeromicrobium, with the name Aeromicrobium halotolerans sp. nov. The type strain is YIM Y47T (=KCTC 39113T=CGMCC 1.15063T=DSM 29939T=JCM 30627T).

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Acknowledgments

The authors are grateful to Prof. Dr. Hans Peter Klenk (DSMZ, Germany) for providing the reference type strains and Prof. Dr. Wen-Jun Li (Yunnan University, China) for experimental guidance. This research work was supported by Key Project of China Ministry of Science and Technology (2013BAD16B05).

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Authors and Affiliations

  1. Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea

    Zheng-Fei Yan, Pei Lin & Tae-Hoo Yi

  2. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Xiao Chu

  3. Department of Marine Biotechnology, Anyang University, Incheon, 417-833, Republic of Korea

    MooChang Kook

  4. Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun, 130118, People’s Republic of China

    Zheng-Fei Yan & Chang-Tian Li

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  1. Zheng-Fei Yan
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  2. Pei Lin
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  3. Xiao Chu
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  4. MooChang Kook
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  5. Chang-Tian Li
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  6. Tae-Hoo Yi
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Correspondence to Chang-Tian Li or Tae-Hoo Yi.

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Communicated by Erko Stackebrandt.

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Yan, ZF., Lin, P., Chu, X. et al. Aeromicrobium halotolerans sp. nov., isolated from desert soil sample. Arch Microbiol 198, 423–427 (2016). https://doi.org/10.1007/s00203-016-1196-6

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  • DOI: https://doi.org/10.1007/s00203-016-1196-6

Keywords

  • Aeromicrobium halotolerans sp. nov.
  • Turpan desert
  • Polyphasic taxonomy