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Bacillus nitroreducens sp. nov., a humus-reducing bacterium isolated from a compost

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Abstract

A Gram-staining-positive, facultative anaerobic, motile and rod-shaped bacterium, designated GSS08T, was isolated from a windrow compost pile and characterized by means of a polyphasic approach. Growth occurred with 0–4 % (w/v) NaCl (optimum 1 %), at pH 6.5–9.5 (optimum pH 7.5) and at 20–45 °C (optimum 37 °C). Anaerobic growth occurred with anthraquinone-2,6-disulphonate, fumarate and NO3 − as electron acceptor. The main respiratory quinone was MK-7. The predominant polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The major fatty acids (>5 %) were iso-C15:0 (43.1 %), anteiso-C15:0 (27.4 %) and iso-C16:0 (8.3 %). The DNA G + C content was 39.6 mol%. The phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GSS08T formed a phyletic lineage with the type strain of Bacillus humi DSM 16318T with a high sequence similarity of 97.5 %, but it displayed low sequence similarity with other valid species in the genus Bacillus (<96.0 %). The DNA–DNA relatedness between strains GSS08T and B. humi DSM 16318T was 50.8 %. The results of phenotypic, chemotaxonomic and genotypic analyses clearly indicated that strain GSS08T represents a novel species, for which the name Bacillus nitroreducens sp. nov. is proposed. The type strain is GSS08T (=KCTC 33699T = MCCC 1K01091T).

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (41203078 and 41301257), Guangdong Natural Science Funds for Distinguished Young Scholar (S20120011151) and the Science and Technology Planning Project of Guangdong Province (2013B060400042 and 2014A030304052).

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Authors and Affiliations

  1. College of Food Science and Technology, Shanghai Ocean University, 999 Huchenghuan Road, Shanghai, 201306, China

    Junhui Guo & Yong Zhao

  2. Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou, 510650, China

    Yue qiang Wang, Guiqin Yang & Li Zhuang

  3. Fuzhou Arboretum, Fuzhou, 350012, China

    Yunqi Chen

  4. College of Resources and Environmental Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Shungui Zhou

Authors
  1. Junhui Guo
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  2. Yue qiang Wang
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  3. Guiqin Yang
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  4. Yunqi Chen
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  5. Shungui Zhou
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  6. Yong Zhao
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  7. Li Zhuang
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Corresponding authors

Correspondence to Yong Zhao or Li Zhuang.

Additional information

Communicated by Erko Stackebrandt.

The GenBank accession numbers for the 16S rRNA gene sequences of strain GSS08T is KT725236.

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Guo, J., Wang, Y.q., Yang, G. et al. Bacillus nitroreducens sp. nov., a humus-reducing bacterium isolated from a compost. Arch Microbiol 198, 347–352 (2016). https://doi.org/10.1007/s00203-016-1193-9

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  • DOI: https://doi.org/10.1007/s00203-016-1193-9

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