Amylibacter ulvae sp. nov., a new alphaproteobacterium isolated from the Pacific green alga Ulva fenestrata

Abstract

A strictly aerobic, Gram-stain-negative, rod-shaped and non-motile bacterium, designated strain 6Alg 255T, was isolated from the green alga Ulva fenestrata. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the novel strain affiliated to the family Rhodobacteraceae of the class Alphaproteobacteria being most closely related to Amylibacter marinus LMG 28364T with 16S rRNA gene sequence similarity of 97.2 %. Strain 6Alg 255T grew with 0.5–6.0 % NaCl and at 4–33 °C, hydrolysed aesculin, casein, gelatin and urea. The DNA G + C content was 50.4 mol%. The prevalent fatty acids were C18:1 ω7c and C16:0. The polar lipid profile was characterized by the presence of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and unidentified aminolipid. The major respiratory quinone was Q-10. The significant molecular distinctiveness between the novel isolate and its nearest neighbour was strongly supported by the differences in physiological and biochemical tests. Therefore, strain 6Alg 255T represents a novel species of the genus Amylibacter, for which the name Amylibacter ulvae sp. nov. is proposed. The type strain is 6Alg 255T (=KCTC 32465T = KMM 6515T).

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Fig. 1

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Acknowledgments

We thank Dr. Stefanie VanTrappen and Claudine Vereecke (BCCM/LMG, Gent, Belgium) for providing us with the type strain Amylibacter marinus LMG 28364T. This research was supported by Grant from the Russian Science Foundation No. 14-14-00030.

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Correspondence to Olga I. Nedashkovskaya.

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Communicated by Erko Stackebrandt.

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Fig. S1

Two-dimensional TLC of the total polar lipids of strain 6Alg 255T (a) and Amylibacter marinus LMG 28364T (b). First dimension, chloroform/methanol/water (65: 25: 4, v/v/v) and second dimension, chloroform/methanol/acetic acid/water (80: 12: 15: 4, v/v/v/v). PE – phosphatidylethanolamine, PG – phosphatidylglycerol, PC – phosphatidylcholine, AL – an unidentified aminolipid. (JPEG 358 kb)

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Nedashkovskaya, O.I., Kukhlevskiy, A.D., Zhukova, N.V. et al. Amylibacter ulvae sp. nov., a new alphaproteobacterium isolated from the Pacific green alga Ulva fenestrata . Arch Microbiol 198, 251–256 (2016). https://doi.org/10.1007/s00203-015-1185-1

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Keywords

  • Amylibacter ulvae sp. nov.
  • Alphaproteobacteria
  • Marine bacteria
  • Phylogeny
  • Green alga Ulva fenestrata