Archives of Microbiology

, Volume 198, Issue 3, pp 251–256 | Cite as

Amylibacter ulvae sp. nov., a new alphaproteobacterium isolated from the Pacific green alga Ulva fenestrata

  • Olga I. NedashkovskayaEmail author
  • Andrey D. Kukhlevskiy
  • Natalia V. Zhukova
  • Seung Bum Kim
Original Paper


A strictly aerobic, Gram-stain-negative, rod-shaped and non-motile bacterium, designated strain 6Alg 255T, was isolated from the green alga Ulva fenestrata. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the novel strain affiliated to the family Rhodobacteraceae of the class Alphaproteobacteria being most closely related to Amylibacter marinus LMG 28364T with 16S rRNA gene sequence similarity of 97.2 %. Strain 6Alg 255T grew with 0.5–6.0 % NaCl and at 4–33 °C, hydrolysed aesculin, casein, gelatin and urea. The DNA G + C content was 50.4 mol%. The prevalent fatty acids were C18:1 ω7c and C16:0. The polar lipid profile was characterized by the presence of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and unidentified aminolipid. The major respiratory quinone was Q-10. The significant molecular distinctiveness between the novel isolate and its nearest neighbour was strongly supported by the differences in physiological and biochemical tests. Therefore, strain 6Alg 255T represents a novel species of the genus Amylibacter, for which the name Amylibacter ulvae sp. nov. is proposed. The type strain is 6Alg 255T (=KCTC 32465T = KMM 6515T).


Amylibacter ulvae sp. nov. Alphaproteobacteria Marine bacteria Phylogeny Green alga Ulva fenestrata 



We thank Dr. Stefanie VanTrappen and Claudine Vereecke (BCCM/LMG, Gent, Belgium) for providing us with the type strain Amylibacter marinus LMG 28364T. This research was supported by Grant from the Russian Science Foundation No. 14-14-00030.

Supplementary material

203_2015_1185_MOESM1_ESM.jpg (359 kb)
Fig. S1 Two-dimensional TLC of the total polar lipids of strain 6Alg 255T (a) and Amylibacter marinus LMG 28364T (b). First dimension, chloroform/methanol/water (65: 25: 4, v/v/v) and second dimension, chloroform/methanol/acetic acid/water (80: 12: 15: 4, v/v/v/v). PE – phosphatidylethanolamine, PG – phosphatidylglycerol, PC – phosphatidylcholine, AL – an unidentified aminolipid. (JPEG 358 kb)
203_2015_1185_MOESM2_ESM.doc (152 kb)
Supplementary material 2 (DOC 152 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Olga I. Nedashkovskaya
    • 1
    • 2
    Email author
  • Andrey D. Kukhlevskiy
    • 2
    • 3
  • Natalia V. Zhukova
    • 2
    • 3
  • Seung Bum Kim
    • 4
  1. 1.G.B. Elyakov Pacific Institute of Bioorganic ChemistryFar-Eastern Branch of the Russian Academy of SciencesVladivostokRussia
  2. 2.Far Eastern Federal UniversityVladivostokRussia
  3. 3.A.V. Zhirmunsky Institute of Marine BiologyFar-Eastern Branch of the Russian Academy of SciencesVladivostokRussia
  4. 4.Department of Microbiology and Molecular Biology, School of Bioscience and BiotechnologyChungnam National UniversityDaejeonRepublic of Korea

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