A Gram-negative, non-motile, non-spore-forming and aerobic bacterium, designated FJ12T, was isolated from seawater, Geoje-si, South Korea. Strain FJ12T was catalase and oxidase positive. Growth of strain FJ12T was optimally observed at 30 °C and pH 6 and grew in the presence of 0–11 % (w/v) NaCl (optimum 2–3 % NaCl). The phylogenic analysis based on 16S rRNA gene sequence of strains FJ12T showed the highest sequence similarity to those of Doghicola eburneus KCTC 12735T (95.4 %), Aestuariihabitans beolgyonensis KTCT 32324T (95.3 %), Sulfitobacter pontiacus KCTC 32185T (94.9 %), Roseisalinus antarcticus DSM 11466T (94.8 %) and Loktanella soesokkaensis KCTC 32425T (94.7 %). The major polar lipids of FJ12T were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, an unidentified aminolipid and unidentified lipids. It contained Q-10 as the predominant ubiquinone, and major fatty acid is C18:1 ω7c (51.7 %), C16:0 (24.0 %). The DNA G+C content of the strain FJ12T was 60 mol%. Based on physiological and chemotaxonomic characteristics, strain FJ12T should be regarded as the type species of a novel genus in the family Rhodobacteraceae for which the name Paradonghicola geojensis sp. nov. is proposed. The type strain FJ12T (=KEMB 3001-336T=JCM 30384T).
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This work was supported by the National Research Foundation of Korea (NRF) (No. NRF-2013M3A2A1067498) and the Korea National Environmental Microorganisms Bank (2010-0007473).
The NCBI GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain FJ12T (=KEMB 3001-336T=JCM 30384T) is KR185328.
Communicated by Erko Stackebrandt.
Electronic supplementary material
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Phylogenetic tree of strains FJ12T and related other type strains based on 16S rRNA gene sequence comparisons. Distances were calculated, and clustering with the maximum-parsimony method was conducted by using the software MEGA5. Bootstrap values of > 50 % (percentages of 1,000 replications) are shown at branching points. The sequences used for the comparative study are included in parentheses (PPTX 115 kb)
Phylogenetic tree of strains FJ12T and related other type strains based on 16S rRNA gene sequence comparisons. Distances were calculated, and clustering with the maximum-likelihood method was conducted by using the software MEGA5. Bootstrap values of > 50 % (percentages of 1,000 replications) are shown at branching points. The sequences used for the comparative study are included in parentheses (PPTX 105 kb)
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Lee, K., Srinivasan, S. & Lee, SS. Paradonghicola geojensis gen. nov., sp. nov., isolated from seawater, Geoje-si, South Korea. Arch Microbiol 198, 501–507 (2016). https://doi.org/10.1007/s00203-015-1182-4
- Paradonghicola geojensis
- Marine bacteria