Abstract
Halophilic archaeal strain XD48T was isolated from a Chinese marine solar saltern. Cells were pleomorphic, stained Gram-negative and formed red-pigmented colonies on solid media. Strain XD48T was found to be able to grow at 25–50 °C (optimum 37 °C), at 0.9–4.8 M NaCl (optimum 3.1 M NaCl), at 0–1.0 M MgCl2 (optimum 0.3 MgCl2) and at pH 5.0–9.5 (optimum pH 6.5). The cells lysed in distilled water, and the minimal NaCl concentration to prevent cell lysis was found to be 5 % (w/v). The major polar lipids of the strain were phosphatidic acid (PA), phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS), sulfated galactosyl mannosyl glucosyl diether (S-TGD-1), sulfated mannosyl glucosyl diether (S-DGD-1) and six unknown glycolipids. The 16S rRNA gene and rpoB′ gene of strain XD48T were phylogenetically related to the corresponding genes of Haloarchaeobius members (92.4–93.9 and 89.6–90.5 % similarities, respectively). The DNA G + C content of strain XD48T was determined to be 65.3 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain XD48T (=CGMCC 1.12230T = JCM 18642T) represents a new species of Haloarchaeobius, for which the name Haloarchaeobius amylolyticus sp. nov. is proposed.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 31370054), the 11th “Six Talents Peak” Project of Jiangsu Province (No. 2014-SWYY-021), the Qinglan Project of Jiangsu Province and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
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Communicated by Harald Huber.
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Phase-contrast micrograph of strain XD48T, thin-layer chromatograms of polar lipids of strain XD48T and some related haloarchaea, neighbor- joining phylogenetic tree reconstructions based on 16S rRNA gene and rpoB′ gene sequences showing the relationships between strain XD48T and related members within the family Halobacteriaceae are available as supplementary materials. (DOC 637 kb)
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Yuan, PP., Sun, XJ., Liang, X. et al. Haloarchaeobius amylolyticus sp. nov., isolated from a marine solar saltern. Arch Microbiol 197, 949–953 (2015). https://doi.org/10.1007/s00203-015-1129-9
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DOI: https://doi.org/10.1007/s00203-015-1129-9