Salisediminibacterium haloalkalitolerans sp. nov., isolated from Lonar soda lake, India, and a proposal for reclassification of Bacillus locisalis as Salisediminibacterium locisalis comb. nov., and the emended description of the genus Salisediminibacterium and of the species Salisediminibacterium halotolerans

Abstract

A Gram stain-positive, rod-shaped, non-motile, orange-pigmented and non-endospore-forming novel bacterial strain 10nlgT was isolated from Lonar soda lake in India. Based on the 16S rRNA gene sequence analysis, it was belonged to the genus Salisediminibacterium and was most closely related to Salisediminibacterium halotolerans CGMCC 1.7654T (99.9 %), Bacillus locisalis CGMCC 1.6286T (99.1 %) and other members in the Bacillaceae (<93.6 %). Further, DNA–DNA hybridization results demonstrated that strain 10nlgT was distantly (<70 %) related to S. halotolerans halo-2T (59.6 %) and B. locisalis CGMCC 1.6286T (53.2 %). Strain 10nlgT was catalase positive and oxidase negative. The cell wall of the strain 10nlgT contains meso-diaminopimelic acid as the diagnostic amino acid. Polar lipids include diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, five phospholipids and two unknown lipids. The predominant isoprenoid quinone was MK-7. Anteiso-C15:0 (32.6 %) was the predominant fatty acid, and significant proportions of iso-C15:0 (9.3 %), anteiso-C17:0 (8.3 %), C16:0 (4.3 %) were also detected in the strain 10nlgT. The DNA G+C content of the strain 10nlgT was 45.6 mol %. The results of phylogenetic, physiological and biochemical tests allowed a distinct differentiation of strain 10nlgT not only from S. halotolerans halo-2T, the only member of the genus Salisediminibacterium, but also from the related Bacillaceae members. Strain 10nlgT represents a novel species of the genus Salisediminibacterium for which the name Salisediminibacterium haloalkalitolerans sp. nov. is proposed. The type strain is 10nlgT (=KCTC 33414T = CGMCC 1.12818T). B. locisalis CGMCC 1.6286T also represents a member of the genus Salisediminibacterium for which the name Salisediminibacterium locisalis CG1T is proposed. The type strain is CG1T = (CCM 7370T = CECT 7152T = CGMCC 1.6286T = DSM 18085T). In accordance with the new polar lipid data collected in this study, emended descriptions of the genus Salisediminibacterium and the species S. halotolerans halo-2T are also provided.

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Acknowledgments

We thank Dr. A. C. Parte for his expert suggestion for correct species epithet and Latin etymology. The authors thank Dr. V.V. Ramana, MCC, NCCS, Pune, India, for his timely help in conducting DNA–DNA hybridization experiments. S.V.R. and M.T. thank DST, Govt. of India, for FTSYS project grants. M.F. also thanks DST for PA fellowship.

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Correspondence to Vishnuvardhan Reddy Sultanpuram.

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The EMBL accession numbers for the 16S rRNA gene sequence of strain 10nlgT is HG934298.

Communicated by Erko Stackebrandt.

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Sultanpuram, V.R., Mothe, T. & Mohammed, F. Salisediminibacterium haloalkalitolerans sp. nov., isolated from Lonar soda lake, India, and a proposal for reclassification of Bacillus locisalis as Salisediminibacterium locisalis comb. nov., and the emended description of the genus Salisediminibacterium and of the species Salisediminibacterium halotolerans . Arch Microbiol 197, 553–560 (2015). https://doi.org/10.1007/s00203-015-1081-8

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Keywords

  • Salisediminibacterium
  • Lonar soda lake
  • Polyphasic taxonomy
  • Salisediminibacterium haloalkalitolerans sp. nov.