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Use of an acidophilic yeast strain to enable the growth of leaching bacteria on solid media

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Abstract

In this study, a Candida digboiensis strain was isolated from a heap leaching plant in Zambia and used in double-layer agar plate to efficiently isolate and purify leaching bacteria. Unlike Acidiphilium sp., the yeast strain was tetrathionate tolerant and could metabolize a great range of organic compounds including organic acids. These properties allowed the yeast strain to enable and fasten the growth of iron and sulfur oxidizers on double-layer agar plate. The isolates were identified as Acidithiobacillus ferrooxidans FOX1, Leptospirillun ferriphilum BN, and Acidithiobacillus thiooxidans ZMB. These three leaching bacteria were inhibited by organic acids such as acetic and propionic acids; however, their activities were enhanced by Candida digboiensis NB under dissolved organic matter stress.

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Acknowledgments

This work was supported by High-Tech Research and Development Program (863 Program: 2012AA061502) and the National Key Basic Research Program of China (973 Program: No. 2010CB630901).

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Authors and Affiliations

  1. School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, Hunan, China

    Baba Ngom, Yili Liang, Yi Liu, Huaqun Yin & Xueduan Liu

  2. Key Laboratory of Biohydrometallurgy, Ministry of Education, Changsha, 410083, Hunan, China

    Baba Ngom, Yili Liang, Yi Liu, Huaqun Yin & Xueduan Liu

Authors
  1. Baba Ngom
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  2. Yili Liang
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  3. Yi Liu
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  4. Huaqun Yin
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  5. Xueduan Liu
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Corresponding author

Correspondence to Baba Ngom.

Additional information

Communicated by Erko Stackebrandt.

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Ngom, B., Liang, Y., Liu, Y. et al. Use of an acidophilic yeast strain to enable the growth of leaching bacteria on solid media. Arch Microbiol 197, 339–346 (2015). https://doi.org/10.1007/s00203-014-1051-6

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  • DOI: https://doi.org/10.1007/s00203-014-1051-6

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