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Mutations in Ralstonia solanacearum loci involved in lipopolysaccharide biogenesis, phospholipid trafficking and peptidoglycan recycling render bacteriophage infection

Archives of Microbiology volume 196, pages 667–674 (2014)Cite this article

Abstract

Ralstonia solanacearum causes deadly wilting on many crops worldwide. However, the information on its components important for cell integrity and interactions with phages is limited. By systematically characterizing mutants resistant to a T7-like phage, we showed that the biosynthesis of rough lipopolysaccharides (R-LPS) was crucial for maintaining the membrane integrity, while the production of smooth LPS (S-LPS) was required for the resistance to polymyxin B and phage adsorption. Furthermore, RSc0154/ampG disruption did not affect LPS production and phage adsorption but may have caused aberrant release of peptidoglycan fragments, thus hindering phage DNA injection into or virion release from the cell. Mutations in the RSc2958–RSc2962/mla cluster, although not affecting LPS production, may have caused elevated phospholipid level in the outer leaflet of the outer membrane, consequently sheltering the mutants from phage adsorption on the O-antigen. These results specify important roles of the biogenesis and homeogenesis of envelope components for R. solanacearum-phage interaction.

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Acknowledgments

We thank the excellent technical assistance of Technology Commons, College of Life Science, National Taiwan University, for the ultracentrifuges (TCX-LS06), the plant green house facility (TCX-E) and quantitative RT-PCR analyses. This work was supported by grants (NSC 98-2311-B-002-009-MY3, NSC 102-2311-B-002-026) from the National Science Council of Taiwan, Republic of China, to C.P.C. This work has been carried out in compliance with local regulations on genetic recombination experimentation.

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  1. Graduate Institute of Plant Biology, National Taiwan University, Taipei, 106, Taiwan, ROC

    Yu-Hau Hong, Chi Huang, Kuan-Chung Wang, Tai-Hsiang Chu, Chien-Hui Li, Yu-Ju Chu & Chiu-Ping Cheng

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  1. Yu-Hau Hong
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  2. Chi Huang
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  3. Kuan-Chung Wang
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  4. Tai-Hsiang Chu
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  5. Chien-Hui Li
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Correspondence to Chiu-Ping Cheng.

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Yu-Hau Hong and Chi Huang have contributed equally to this work.

Communicated by Erko Stackebrandt.

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Hong, YH., Huang, C., Wang, KC. et al. Mutations in Ralstonia solanacearum loci involved in lipopolysaccharide biogenesis, phospholipid trafficking and peptidoglycan recycling render bacteriophage infection. Arch Microbiol 196, 667–674 (2014). https://doi.org/10.1007/s00203-014-1002-2

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  • DOI: https://doi.org/10.1007/s00203-014-1002-2

Keywords

  • Ralstonia solanacearum
  • Bacteriophage
  • Lipopolysaccharide
  • Peptidoglycan
  • Phospholipid