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Transcriptome analysis of Enterococcus faecalis toward its adaption to surviving in the mouse intestinal tract

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Abstract

We have performed a transcriptomic in vivo study with Enterococcus faecalis OG1RF in the intestine of living mice to identify novel latent and adaptive fitness determinants within E. faecalis. From 2,658 genes that are present in E. faecalis strain OG1RF, 124 genes were identified as significantly differentially expressed within the intestinal tract of living mice as compared to exponential growth in BHI broth. The groups of significantly up- or down-regulated genes consisted of 94 and 30 genes, respectively, for which 46 and 18 a clear annotation to a functionally described protein was found. These included genes involved in energy metabolism (e.g., dhaK and glpK pathway), transport and binding mechanisms (e.g., phosphoenolpyruvate carbohydrate PTS) as well as fatty acid metabolism (fab genes). The novel putative fitness determinants found in this work may be helpful for future studies of E. faecalis adaptation to the intestinal tract, which is also a prerequisite for infection in a compromised or inflamed host.

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Acknowledgments

We thank Dr. Sigrid Kisling (Chair for Biofunctionality of Food, Technische Universität München, Germany) for histology analysis of mouse tissue samples used in this study. We also thank the members of the National Gnotobiotic Rodent Resource Center (University of North Carolina, Chapel Hill, USA) for the generous support during the experiment. This work was supported by GRK 1482 of the German Research Foundation (DFG), NIH grants R01DK53247, P40 OD010995, P30 DK34987 and the Crohn’s and Colitis Foundation of America. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.

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Correspondence to Rudi F. Vogel.

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Communicated by Erko Stackebrandt.

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Lindenstrauß, A.G., Ehrmann, M.A., Behr, J. et al. Transcriptome analysis of Enterococcus faecalis toward its adaption to surviving in the mouse intestinal tract. Arch Microbiol 196, 423–433 (2014). https://doi.org/10.1007/s00203-014-0982-2

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