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Identification of 4″-isovaleryl-spiramycin III produced by Bacillus sp. fmbJ

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Abstract

The production of secondary metabolites with antibiotic properties is a common characteristic to Bacillus spp. These metabolites not only have diverse chemical structures but also have a wide range of bioactivities with medicinal and agricultural interests such as antibiotic. Bacillus sp. fmbJ has been found to produce lipopeptides fengycin and surfactin in accordance with our previous report. In this study, another antimicrobial substance was separated and purified from the culture supernatant of strain fmbJ using the silica gel column chromatography and preparative reversed-phase high-performance liquid chromatography. By means of electrospray ionization mass spectroscopy, infrared spectroscopy, and nuclear magnetic resonance, the antagonistic compound was determined to be 4″-isovaleryl-spiramycin III with the molecular weight of 982 Da. This report is the first to introduce the finding of spiramycin produced from Bacillus sp. The study provides a novel source for the production of spiramycin in pharmaceutical industries.

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References

  • Benslimane C, Lebrihi A, Lounès A, Lefebvre G, Germain P (1995) Influence of dextrins on the assimilation of yeast extract amino acids in culture of Streptomyces ambofaciens producer of spiramycin. Enzyme Microb Technol 17:1003–1013

    Article  CAS  Google Scholar 

  • Besson F, Hourdou ML, Michel G (1990) Studies on the biosynthesis of iturin, an antibiotic of Bacillus subtilis, and a lipopeptide containing beta-hydroxy fatty acids. Biochim Biophys Acta 1036:101–106

    Article  CAS  PubMed  Google Scholar 

  • Bie XM, Lu ZX, Lu FX, Zeng XX (2005) Screening the main factors affecting extraction of the antimicrobial substance from Bacillus sp. fmbJ using the Plackett-Burman method. World J Microbiol Biotechnol 21:925–928

    Article  CAS  Google Scholar 

  • Bie XM, Lu ZX, Lu FX (2009) Identification of fengycin homologues from Bacillus subtilis with ESI-MS/CID. J Microbiol Meth 79:272–278

    Article  CAS  Google Scholar 

  • Brötz H, Bierbaum G, Reynolds PE, Sahl HG (1997) The lantibiotic mersacidin inhibits peptidoglycan biosynthesis at the level of transglycosylation. Eur J Biochem 246:193–199

    Article  PubMed  Google Scholar 

  • Deng Y, Lu ZX, Lu FX, Wang Y, Bie XM (2011a) Study on an antimicrobial protein produced by Paenibacillus polymyxa JSa-9 isolated from soil. World J Microbiol Biotechnol 27:1803–1807

    Article  CAS  Google Scholar 

  • Deng Y, Lu ZX, Lu FX, Zhang C, Wang Y, Zhao HZ, Bie XM (2011b) Identification of LI-F type antibiotics and di-n-butyl phthalate produced by Paenibacillus polymyxa. J Microbiol Meth 85:175–182

    Article  CAS  Google Scholar 

  • Deng Y, Lu ZX, Bi H, Lu FX, Zhang C, Bie XM (2011c) Isolation and characterization of peptide antibiotics LI-F04 and polymyxin B6 produced by Paenibacillus polymyxa strain JSa-9. Peptides 32:1917–1923

    Article  CAS  PubMed  Google Scholar 

  • Duitman EH, Hamoen LW, Rembold M, Venema G, Seitz H, Saenger W, Bernhard F, Reinhardt R, Schmidt M, Ullrich C, Stein T, Leenders F, Vater J (1999) The mycosubtilin synthetase of Bacillus subtilis ATCC6633: a multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase. Proc Natl Acad Sci USA 96:13294–13299

    Article  CAS  PubMed  Google Scholar 

  • Hu M, Hu CQ (2005) Identification of the components of 16-membered macrolide antibiotics by LC/MS. Anal Chim Acta 535:89–99

    Article  CAS  Google Scholar 

  • Inaoka T, Takahashi K, Ohnishi-Kameyama M, Yoshida M, Ochi K (2003) Guanine nucleotides guanosine 5′-diphosphate 3′-diphosphate and GTP co-operatively regulate the production of an antibiotic bacilysin in Bacillus subtilis. J Biol Chem 278:2169–2176

    Article  CAS  PubMed  Google Scholar 

  • Joshi S, Bharucha C, Desai AJ (2008) Production of biosurfactant and antifungal compound by fermented food isolate Bacillus subtilis 20B. Bioresour Technol 99:4603–4608

    Article  CAS  PubMed  Google Scholar 

  • Klein C, Entian KD (1994) Genes involved in self-protection against the lantibiotic subtilin produced by Bacillus subtilis ATCC 6633. Appl Environ Microbiol 60:2793–2801

    CAS  PubMed Central  PubMed  Google Scholar 

  • Landy M, Warren GH, Rosenman SB, Colio LG (1948) An antibiotic from Bacillus subtilis active against pathogenic fungi. Proc Soc Exp Med 67:539–541

    Article  CAS  Google Scholar 

  • Lee YK, Senthilkumar M, Kim JH, Swarnalakshmi K, Annapurna K (2008) Purification and partial characterization of antifungal metabolite from Paenibacillus lentimorbus WJ5. World J Microbiol Biotechnol 24:1591–1597

    Google Scholar 

  • Liu YF, Sun CH, Jin WZ (1997) Isolation and structural identification of shengjimycin A and B, the major components of shengjimycins (in Chinese). Chin J Antibiot 5:328–333

    Google Scholar 

  • Moyne AL, Cleveland TE, Tuzun S (2004) Molecular characterization and analysis of the operon encoding the antifungal lipopeptide bacillomycin D. FEMS Microbiol Lett 234:43–49

    Article  CAS  PubMed  Google Scholar 

  • Murray EJ, Stanley-Wall NR (2010) The sensitivity of Bacillus subtilis to diverse antimicrobial compounds is influenced by Abh. Arch Microbiol 192:1059–1067

    Article  CAS  PubMed  Google Scholar 

  • Nakayma S, Takahashi M, Hirai M, Shoda M (1997) Isolation of a new variants of surfactin by a recombinant Bacillus subtilis. Appl Microbiol Biotechnol 48:80–82

    Article  Google Scholar 

  • Paik SH, Chakicherla A, Hansen JN (1998) Identification and characterization of the structural and transporter genes for, and the chemical and biological properties of, sublancin 168, a novel lantibiotic produced by Bacillus subtilis 168. J Biol Chem 273:23134–23142

    Article  CAS  PubMed  Google Scholar 

  • Pinnert-Sindico S, Ninet L, Preud’Homme J, Cosar C (1955) A new antibiotic-spiramycin. Antibiot Annu 1954–1955:724–727

    Google Scholar 

  • Shi D, Kong Y, Yu J, Wang Y, Yang J (2006) Separation performance of polyimide nanofiltration membranes for concentrating spiramycin extract. Desalination 191:309–317

    Article  CAS  Google Scholar 

  • Si W, Gong J, Tsao R, Kalab M, Yang R, Yin Y (2006) Bioassay-guided purification and identification of antimicrobial components in Chinese green tea extract. J Chromatogr A 1125:204–210

    Article  CAS  PubMed  Google Scholar 

  • Stein T (2005) Bacillus subtilis antibiotics: structures, syntheses and specific functions. Mol Microbiol 56:845–857

    Article  CAS  PubMed  Google Scholar 

  • Stein T, Borchert S, Conrad B, Feesche J, Hofemeister B, Hofemeister J, Entian KD (2002) Two different lantibiotic-like peptides originate from the ericin gene cluster of Bacillus subtilis A1/3. J Bacteriol 184:1703–1711

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Vanittanakom N, Loeffler W, Koch U, Jung G (1986) Fengycin-a novel antifungal lipopeptide antibiotic produced by Bacillus subtilis F-29-3. J Antibiot 39:888–901

    Article  CAS  PubMed  Google Scholar 

  • Zheng G, Yan LZ, Vederas JC, Zuber P (1999) Genes of the sbo-alb locus of Bacillus subtilis are required for production of the antilisterial bacteriocin subtilosin. J Bacteriol 181:7346–7355

    CAS  PubMed Central  PubMed  Google Scholar 

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Acknowledgments

We would like to thank the National Natural Science Foundation of China for their financial support (Grant No. 30671460 and No. 30871753).

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Authors and Affiliations

  1. College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture of China, 1 Weigang, Nanjing, 210095, China

    Yang Deng, Yanjuan Ju, Zhaoxin Lu, Fengxia Lu, Dong Yan & Xiaomei Bie

Authors
  1. Yang Deng
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  2. Yanjuan Ju
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  3. Zhaoxin Lu
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  4. Fengxia Lu
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  5. Dong Yan
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  6. Xiaomei Bie
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Corresponding author

Correspondence to Xiaomei Bie.

Additional information

Communicated by Erko Stackebrandt.

Yang Deng and Yanjuan Ju have contributed equally to this paper.

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Deng, Y., Ju, Y., Lu, Z. et al. Identification of 4″-isovaleryl-spiramycin III produced by Bacillus sp. fmbJ. Arch Microbiol 196, 87–95 (2014). https://doi.org/10.1007/s00203-013-0939-x

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  • DOI: https://doi.org/10.1007/s00203-013-0939-x

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