Required characteristics of Paenibacillus polymyxa JB-0501 as potential probiotic
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The ability of Paenibacillus polymyxa to inhibit the growth of Escherichia coli generic ATCC 25922 (Escherichia coli ATCC 25922) and to adhere to monolayers of the enterocyte-like human cell line Caco-2 was evaluated. P. polymyxa JB-0501 (P. polymyxa JB-0501), found in a livestock feed probiotic supplement, was compared to P. polymyxa reference strain ATCC 43685 and ATCC 7070 (P. polymyxa ATCC) in terms of carbohydrate utilization and resistance to lysozyme, acid, bile salts, and hydrogen peroxide. JB-0501 grew at pH 4.5 and at H2O2 concentrations less than 7.3 μg/ml and presented a higher affinity to hexadecane and decane. Bile salts at 0.2 % inhibited the growth of all three strains. P. polymyxa JB-0501 and P. polymyxa ATCC 43865 adhered to Caco-2 cell monolayers. The percentage of cells that adhered ranged from about 0.35 to 6.5 % and was partially proportional to the number applied. Contact time (from 15 min to 1 h) had little impact on adhesion. P. polymyxa JB-0501 inhibited the growth of E. coli ATCC 25922, as proven by the diffusion tests in agar. Taken together, these results suggested that P. polymyxa JB-0501 has the potential probiotic properties to justify its consideration as a livestock feed supplement.
KeywordsPaenibacillus polymyxa JB-0501 Escherichia coli Caco-2 cells Adhesion Pathogenic bacteria Probiotic properties
This research was supported by Best Environmental Technologies Inc., Edmonton, AB, Canada.
- Charteris WP, Kelly PM, Morelli L, Collins JK (1998) Development and application of an in vitro methodology to determine the transit tolerance of potentially probiotic Lactobacillus and Bifidobacterium species in the upper human gastrointestinal tract. J Appl Microbiol 84:759–768PubMedCrossRefGoogle Scholar
- Lankaputhra EV, Shah NP (1995) Survival of Lactobacillus acidophilus and Bifidobacterium spp. in the presence of acid and bile salts. Cult Dairy Prod J 30:2–7Google Scholar
- Suskovic J, Brkic B, Matosic S, Maric V (1997) Lactobacillus acidophilus M92 as potential probiotic strain. Milchwissenschaft 52:430–435Google Scholar