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Colonization of Madagascar periwinkle (Catharanthus roseus), by endophytes encoding gfp marker

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Abstract

This study reports the introduction of gfp marker in two endophytic bacterial strains (Pantoea agglomerans C33.1, isolated from cocoa, and Enterobacter cloacae PR2/7, isolated from citrus) to monitor the colonization in Madagascar perinwinkle (Catharanthus roseus). Stability of the plasmid encoding gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under non-selective conditions. The colonization was observed using fluorescence microscopy and enumeration of culturable endophytes in inoculated perinwinkle plants that grew for 10 and 20 days. Gfp-expressing strains were re-isolated from the inner tissues of surface-sterilized roots and stems of inoculated plants, and the survival of the P. agglomerans C33:1gfp in plants 20 days after inoculation, even in the absence of selective pressure, suggests that is good colonizer. These results indicated that both gfp-tagged strains, especially P. agglomerans C33.1, may be useful tools to deliver enzymes or other proteins in plant.

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Acknowledgments

Funding for this research was provided by FAPESP. A. R. Torres and M. Mondin are CAPES fellows. P. Rossetto and W. L. Araújo are FAPESP fellows. L. Cursino, M. Hungria, and J. L. Azevedo are CNPq fellows. The authors thank M. C. Quecine for help in statistical analysis and Drs. Glaciela Kaschuk and Paulo Lacava for suggestions on the manuscript.

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Author notes

  1. Welington Luiz Araújo

    Present address: Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374-Ed. Biomédicas II, Cidade Universitária, São Paulo, São Paulo, 05508-900, Brazil

  2. Luciana Cursino

    Present address: Department of Biology, Hobart and William Smith Colleges, 300 Pulteney Street, Geneva, NY, USA

  3. Priscilla de Barros Rossetto

    Present address: Instituto de Biologia, Departamento de Genética, Universidade Federal do Rio de Janeiro, Av. Prof. Rodolpho Paulo Rocco s/n Prédio do CCS, 2° Andar, Sala A2-93, Ilha do Fundão, Rio de Janeiro, RJ, 21949-900, Brazil

Authors and Affiliations

  1. Departamento de Genética, Escola Superior de Agricultura ‘Luiz de Queiroz’, Universidade de São Paulo, PO Box 83, Piracicaba, São Paulo, 13400-970, Brazil

    Adalgisa Ribeiro Torres, Luciana Cursino, Mateus Mondin & João Lúcio Azevedo

  2. Embrapa Soja, PO Box 231, Londrina, Paraná, 86001-970, Brazil

    Adalgisa Ribeiro Torres & Mariangela Hungria

Authors
  1. Adalgisa Ribeiro Torres
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  2. Welington Luiz Araújo
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  3. Luciana Cursino
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  4. Priscilla de Barros Rossetto
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  5. Mateus Mondin
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  6. Mariangela Hungria
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  7. João Lúcio Azevedo
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Corresponding author

Correspondence to Adalgisa Ribeiro Torres.

Additional information

Communicated by Erko Stackebrandt.

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Torres, A.R., Araújo, W.L., Cursino, L. et al. Colonization of Madagascar periwinkle (Catharanthus roseus), by endophytes encoding gfp marker. Arch Microbiol 195, 483–489 (2013). https://doi.org/10.1007/s00203-013-0897-3

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  • DOI: https://doi.org/10.1007/s00203-013-0897-3

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