Abstract
In many wild legumes, attempts to cultivate nodule bacteria fail. We hypothesized that the limited culturability could be related to injury from oxidative stress caused by disruption of plant tissues during isolation. To test that, we isolated bacteria from nodules of Hedysarum spinosissimum and Tetragonolobus purpureus using buffers supplemented with scavenging systems to prevent damage from reactive oxygen species (ROS). Treatments included the following: antioxidants (glutathione, ascorbate, EDTA) or enzymes (catalase, peroxidase, superoxide dismutase), tested either as modified squashing buffers or added in plates. Some combinations yielded dramatic increases of culturability. Different endophytes were found, including additional Rhizobiaceae that were not the primary symbiont and were unable to nodulate. Their H2O2 tolerance in broth culture showed differences consistent with the unequal culturability observed. In wild legumes species, ROS generation during extraction appears to be a major factor limiting microbiota isolation, and protocols presented here significantly improve the recovery of culturable bacterial endophytes from plants.
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Acknowledgments
We thank Piero Cappuccinelli for stimulating discussions. This work was supported in part by a grant from Fondazione Banco di Sardegna 2010 p1293/2010.1163 to RM and by Ex-60 % funds of the University of Padova to AS and BB. AT. was supported by an Assegno di Ricerca grant of the University of Padova # CPDR093189.
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Communicated by Jörg Overmann.
Rosella Muresu and Alessandra Tondello: equal contributors.
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Muresu, R., Tondello, A., Polone, E. et al. Antioxidant treatments counteract the non-culturability of bacterial endophytes isolated from legume nodules. Arch Microbiol 195, 385–391 (2013). https://doi.org/10.1007/s00203-013-0886-6
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DOI: https://doi.org/10.1007/s00203-013-0886-6