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Diversity of the major capsid genes (g23) of T4-like bacteriophages in the eutrophic Lake Kotokel in East Siberia, Russia

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Abstract

Numerous studies revealed high diversity of T4-like bacteriophages in various environments, but so far, little is known about T4-like virus diversity in freshwater bodies, particularly in eutrophic lakes. The present study was aimed at elucidating molecular diversity of T4-like bacteriophages in eutrophic Lake Kotokel located near Lake Baikal by partial sequencing of the major capsid genes (g23) of T4-like bacteriophages. The majority of g23 fragments from Lake Kotokel were most similar to those from freshwater lakes and paddy fields. Despite the proximity and direct water connection between Lake Kotokel and Lake Baikal, g23 sequence assemblages from two lakes were different. UniFrac analysis showed that uncultured T4-like viruses from Lake Kotokel tended to cluster with those from the distant lake of the same trophic status. This fact suggested that the trophic conditions affected the formation of viral populations, particularly of T4-like viruses, in freshwater environments.

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Acknowledgments

We are grateful to Dr. Tatyana Sherbakova (Limnological Institute, SB RAS), who helped in execution of the paper. This study was supported by the RFBR, research project nos. 10-04-01613, 11-04-92220, and by the SB RAS Project, no. 55.

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Authors and Affiliations

  1. Limnological Institute, Siberian Branch of Russian Academy of Sciences, P.O. Box 278, 664033, Irkutsk, Russia

    Tatyana V. Butina, Olga I. Belykh, Sergey A. Potapov & Ekaterina G. Sorokovikova

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  1. Tatyana V. Butina
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  2. Olga I. Belykh
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  3. Sergey A. Potapov
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  4. Ekaterina G. Sorokovikova
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Correspondence to Tatyana V. Butina.

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Communicated by Shuang-Jiang Liu.

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Butina, T.V., Belykh, O.I., Potapov, S.A. et al. Diversity of the major capsid genes (g23) of T4-like bacteriophages in the eutrophic Lake Kotokel in East Siberia, Russia. Arch Microbiol 195, 513–520 (2013). https://doi.org/10.1007/s00203-013-0884-8

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  • DOI: https://doi.org/10.1007/s00203-013-0884-8

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