Abstract
The effect of intestinal molecules produced by the host on the virulence of Pseudomonas fluorescens is poorly documented. In the present work, we evaluated the secretion of human β-defensin-2 (hBD-2) by enterocytes after infection with P. fluorescens (a species previously suggested to be involved in inflammatory bowel disease) and investigated the effect of this host-defense peptide on the bacterial virulence. The results showed that P. fluorescens can induce hBD-2 production in Caco-2/TC7 cells via P38 and ERK MAPK-dependent pathways. Surprisingly, the exposure of P. fluorescens to low doses of the antimicrobial peptide was found to enhance its cytotoxic and proinflammatory effects suggesting a potential feedback mechanism in the dialog between bacteria and the host.
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Acknowledgments
This study was supported by grants from the Conseil Général de l’Eure, the Communauté d’Agglomération d’Evreux and Europe. LMSM is a member and supported by the world’s leading center Cosmetic Valley.
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Communicated by Djamel Drider.
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Madi, A., Alnabhani, Z., Leneveu, C. et al. Pseudomonas fluorescens can induce and divert the human β-defensin-2 secretion in intestinal epithelial cells to enhance its virulence. Arch Microbiol 195, 189–195 (2013). https://doi.org/10.1007/s00203-012-0865-3
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DOI: https://doi.org/10.1007/s00203-012-0865-3