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Archives of Microbiology

, Volume 195, Issue 2, pp 141–144 | Cite as

Molecular characterization of extended-spectrum-beta-lactamase-producing Escherichia coli isolates from red foxes in Portugal

  • Hajer Radhouani
  • Gilberto Igrejas
  • Alexandre Gonçalves
  • Vanesa Estepa
  • Roberto Sargo
  • Carmen Torres
  • Patrícia PoetaEmail author
Short Communication

Abstract

The presence of broad-spectrum-cephalosporin-resistant Escherichia coli isolates and the implicated mechanisms of resistance and virulence factor genes were investigated in red fox (Vulpes vulpes) in Portugal. Cefotaxime-resistant E. coli isolates were isolated from two of 52 fecal samples (4 %), being both ESBL producers. The β-lactamase genes found in the two isolates were bla SHV-12 + bla TEM-1b. The tet(A) and sul2 genes were also detected in these isolates, together with the non-classical class 1 integron (intI1-dfrA12-orfF-aadA2-cmlA1-aadA1-qacH-IS440-sul3) with the PcH1 promoter. The two isolates belonged to the phylogroup A. Amino acid changes in GyrA (S83L + D87G) and ParC (S80I) proteins were identified in our study. Concerning MLST typing, both isolates were assigned to ST1086, never found before in wild animals, and they presented closely related PFGE patterns. This study reveals the presence of ESBL-producing E. coli isolates, in a wild ecosystem, which could be disseminated through the environment to other niches.

Keywords

ESBLs Escherichia coli Phylogenetic groups Vulpes vulpes Virulence factors 

Notes

Acknowledgments

We thank the forest brigade of the General Corporation of Forestry Resources and CRATAS (Center of Collection, Welcome and Handling of Wild Animals) for their contribution to the collection of samples. Hajer Radhouani and Alexandre Gonçalves both were granted by Fundação para a Ciência e a Tecnologia (FCT) from Portugal with reference SFRH/BD/60846/2009 and SFRH/BD/47833/2008, respectively. Vanesa Estepa has a fellowship from the University of La Rioja (FPI-UR-09/16599009R).

Supplementary material

203_2012_853_MOESM1_ESM.doc (88 kb)
Supplementary material 1 (DOC 88 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Hajer Radhouani
    • 1
    • 2
    • 3
    • 4
  • Gilberto Igrejas
    • 1
    • 2
  • Alexandre Gonçalves
    • 1
    • 2
    • 3
    • 4
  • Vanesa Estepa
    • 5
  • Roberto Sargo
    • 6
  • Carmen Torres
    • 5
  • Patrícia Poeta
    • 3
    • 4
    Email author
  1. 1.Institute for Biotechnology and Bioengineering, Center of Genomics and BiotechnologyUniversity of Trás-os-Montes and Alto DouroVila RealPortugal
  2. 2.Department of Genetics and BiotechnologyUniversity of Trás-os-Montes and Alto DouroVila RealPortugal
  3. 3.Center of Studies of Animal and Veterinary SciencesVila RealPortugal
  4. 4.Veterinary Science DepartmentUniversity of Trás-os-Montes and Alto DouroVila RealPortugal
  5. 5.Biochemistry and Molecular Biology AreaUniversity of La RiojaLogroñoSpain
  6. 6.CRATASUniversity of Trás-os-Montes and Alto DouroVila RealPortugal

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