Skip to main content
SpringerLink
Log in

Pseudomonas nitritireducens sp. nov., a nitrite reduction bacterium isolated from wheat soil

  • Mini-Review
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

A Gram-negative, non-mobile, polar single flagellum, rod-shaped bacterium WZBFD3-5A2T was isolated from a wheat soil subjected to herbicides for several years. Cells of strain WZBFD3-5A2T grow optimally on Luria-Bertani agar medium at 30 °C in the presence of 0–4.0 % (w/v) NaCl and pH 8.0. 16S rRNA gene sequence analysis revealed that strain WZBFD3-5A2T belongs to the genus Pseudomonas. Physiological and biochemical tests supported the phylogenetic affiliation. Strain WZBFD3-5A2T is closely related to Pseudomonas nitroreducens IAM1439T, sharing 99.7 % sequence similarity. DNA–DNA hybridization experiments between the two strains showed only moderate reassociation similarity (33.92 ± 1.0 %). The DNA G+C content is 62.0 mol%. The predominant respiratory quinine is Q-9. The major cellular fatty acids present are C16:0 (28.55 %), C16:1ω6c or C16:1ω7c (20.94 %), C18:1ω7c (17.21 %) and C18:0 (13.73 %). The isolate is distinguishable from other related members of the genus Pseudomonas on the basis of phenotypic and biochemical characteristics. From the genotypic, chemotaxonomic and phenotypic data, it is evident that strain WZBFD3-5A2T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas nitritereducens sp. nov. is proposed. The type strain is WZBFD3-5A2T (=CGMCC 1.10702T = LMG 25966T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    PubMed  CAS  Google Scholar 

  • Andrews JM (2008) BSAC standardized disc susceptibility testing method (Version 7). J Antimicrob Chemother 62:256–278

    Article  PubMed  CAS  Google Scholar 

  • Cappuccino JG, Sherman N (2002) Microbiology: a Laboratory Manual, 6th edn. Pearson Education, Inc. and Benjamin Cummings, San Francisco

    Google Scholar 

  • Chun J, Lee JH, Jung Y, Kim M, Kim S, Kim BK, Lim YW (2007) EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261

    Article  PubMed  CAS  Google Scholar 

  • Clarke PH (1953) Hydrogen sulphide production by bacteria. J Gen Microbiol 8:397–407

    Article  PubMed  CAS  Google Scholar 

  • De Ley J, Cattoir H, Reynaerts A (1970) The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142

    Article  PubMed  Google Scholar 

  • Denner EB, Paukner S, Kampfer P, Moore ER, Abraham WR, Busse HJ, Wanner G, Lubitz W (2001) Description of Sphingomonas pituitosa sp. nov., a novel polysaccharide-producing sphingomonad. Int J Syst Evol Microbiol 51:827–841

    Article  PubMed  CAS  Google Scholar 

  • Dong XZ, Cai MY (2001) Determinative manual for routine bacteriology. Scientific Press, Beijing (in Chinese)

    Google Scholar 

  • Embley TM (1991) The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. Lett Appl Microbiol 13:171–174

    Article  PubMed  CAS  Google Scholar 

  • Fraser SL, Jorgensen JH (1997) Reappraisal of the antimicrobial susceptibilities of Chtyseobacterium and Flavobacterium species and methods for reliable susceptibility testing. Antimicrob Agents Chemother 41:2738–2741

    PubMed  CAS  Google Scholar 

  • Gerhardt P, Murray RGE, Wood WA, Krieg NR (1994) Methods for General and Molecular Bacteriology. American Society for Microbiology, Washington, DC

    Google Scholar 

  • Gupta SK, Kumari R, Prakash O, Lal R (2008) Pseudomonas panipatensis sp. nov., isolated from an oil-contaminated site. Int J Syst Evol Microbiol 58:1339–1345

    Article  PubMed  CAS  Google Scholar 

  • Hiraishi A, Ueda Y, Ishihara J (1998) Quinone profiling of bacterial communities in natural and synthetic sewage activated sludge for enhanced phosphate removal. Appl Environ Microbiol 64:992–998

    PubMed  CAS  Google Scholar 

  • Johnson JL (1994) Similarity analysis of DNAs. In: Gerhardt PE, Murray RG, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, pp 655–681

    Google Scholar 

  • Kwon SW, Kim JS, Park IC, Yoon SH, Park DH, Lim CK, Go SJ (2003) Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 53:21–27

    Article  PubMed  CAS  Google Scholar 

  • Lang E, Griese B, Spröer C, Schumann P, Steffen M, Verbarg S (2007) Characterization of ‘Pseudomonas azelaica’ DSM 9128, leading to emended descriptions of Pseudomonas citronellolis Seubert 1960 (Approved Lists 1980) and Pseudomonas nitroreducens Iizuka and Komagata 1964 (Approved Lists 1980), including Pseudomonas multiresinivorans as its later heterotypic synonym. Int J Syst Evol Microbiol 57:878–882

    Article  PubMed  CAS  Google Scholar 

  • Las Heras A, Domínguez L, López I, Fernández-Garayzábal JF (1999) Outbreak of acute ovine mastitis associated with Pseudomonas aeruginosa infection. Vet Rec 145:111–112

    Article  PubMed  CAS  Google Scholar 

  • Liu M, Luo XS, Zhang L, Dai J, Wang Y, Tang YL, Li J, Sun TJ, Fang CX (2009) Pseudomonas xinjiangensis sp. nov., a moderately thermotolerant bacterium isolated from desert sand. Int J Syst Evol Microbiol 59:1286–1289

    Article  PubMed  CAS  Google Scholar 

  • Mandel M, Igambi L, Bergenda J, Dodson ML, Scheltge E (1970) Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. J Bacteriol 101:333–338

    PubMed  CAS  Google Scholar 

  • Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218

    Article  CAS  Google Scholar 

  • Mata JA, Martínez-Cánovas J, Quesada E, Béjar V (2002) A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 25:360–375

    Article  PubMed  CAS  Google Scholar 

  • MIDI (2005) Sherlock Microbial Identification System Operating Manual, version 6.0. Newark DE: MIDI Inc

  • Migula W (1894) Uber ein neues System der Bakterien. Arb Bakteriol Inst Karlsruhe 1:235–238

    Google Scholar 

  • Palleroni NJ (1984) Genus I. Pseudomonas Migula 1894, 237AL (nom. cons. Opin. 5, Jud. Comm. 1952, 237). In: Krieg NR, Holt JG (eds) Bergey’s Manual of Systematic Bacteriology, vol. 1. Williams & Wilkins, Baltimore, pp 141–199

  • Prakash O, Kumari K, Lal R (2007) Pseudomonas delhiensis sp. nov., from a fly ash dumping site of a thermal power plant. Int J Syst Evol Microbiol 57:527–531

    Article  PubMed  CAS  Google Scholar 

  • Qin QL, Zhao DL, Wang J, Chen XL, Dang HY, Li TG, Zhang YZ, Gao PJ (2007) Wangia profunda gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from southern Okinawa trough deep-sea sediment. FEMS Microbiol Lett 271:53–58

    Article  PubMed  CAS  Google Scholar 

  • Romano I, Lama L, Nicolaus B, Poli A, Gambacorta A, Giordano A (2006) Halomonas alkaliphila sp. nov., a novel halotolerant alkaliphilic bacterium isolated from a salt pool in Campania (Italy). J Gen Appl Microbiol 52:339–348

    Article  PubMed  CAS  Google Scholar 

  • Stanier RY, Palleroni NJ, Doudoroff M (1966) The aerobicpseudomonads: a taxonomic study. J Gen Microbiol 43:159–271

    Article  PubMed  CAS  Google Scholar 

  • Stolz A, Busse HJ, Kämpfer P (2007) Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57:572–576

    Article  PubMed  CAS  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  PubMed  CAS  Google Scholar 

  • Wang YN, Cai H, Yu SL, Wang ZY, Wu XL (2007) Halomonas gudaonensis sp. nov., isolated from a saline soil contaminated by crude oil. Int J Syst Evol Microbiol 57:911–915

    Article  PubMed  CAS  Google Scholar 

  • Wu JF, Shen XH, Zhou YG, Liu SJ (2004) Characterization of p-chloronitrobenzene-degrading Comamonas sp. CNB1 and its degradation of p-chloronitrobenzene. Acta Microbiol Sinica 44:8–12

    Google Scholar 

  • Zumft WG (1992) The denitrifying bacteria. In: Balows A, Truper HG, Dworkin M, Harder W, Schleifer KH (eds) The Prokaryotes, 2nd edn. Springer, New York, pp 554–582

    Google Scholar 

Download references

Acknowledgments

The authors thank Professor De-Rong An (Shanxi Key Laboratory of Molecular Biology for Agriculture, College of Plant Protection, Northwest Agricultural & Forestry University, Yanglin, China) for providing the transmission electron microscopy facility and Professor Xiao-Jun Yan (Ningbo University, China) for excellent technical assistance. This work was supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant: KSCX2-YW-G-052) and by the National Natural Science Foundation of China (Grant: 31100582).

Author information

Authors and Affiliations

  1. Key Laboratory of Microbial Engineering at the Institute of Biology, Henan Academy of Sciences, Zhengzhou, 450008, People’s Republic of China

    Ya-Nan Wang, Wei-Hong He, Hua He, Xun Du, Bin Jia, Zhi-Peng Zeng, Ming-Li An & Guo-Can Chen

  2. College of Bioengineering, Henan University of Technology, Henan, Zhengzhou, 450001, People’s Republic of China

    Hua He

Authors
  1. Ya-Nan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wei-Hong He
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hua He
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xun Du
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bin Jia
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Zhi-Peng Zeng
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ming-Li An
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Guo-Can Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ya-Nan Wang.

Additional information

Communicated by Erko Stackebrandt.

Ya-Nan Wang and Wei-Hong He contributed equally to this work.

Sequence deposited: The GenBank accession number for the 16S rRNA gene sequence of strain WZBFD3-5A2T is HM246143.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 87 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, YN., He, WH., He, H. et al. Pseudomonas nitritireducens sp. nov., a nitrite reduction bacterium isolated from wheat soil. Arch Microbiol 194, 809–813 (2012). https://doi.org/10.1007/s00203-012-0838-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00203-012-0838-6

Keywords

Search

Navigation