Archives of Microbiology

, Volume 194, Issue 6, pp 415–426 | Cite as

The biosynthesis of the polyether antibiotic nanchangmycin is controlled by two pathway-specific transcriptional activators

Original Paper

Abstract

The nanchangmycin (NAN) produced by Streptomyces nanchangensis NS3226 is a polyether antibiotic resembling monensin in their gene clusters and the chemical structures. They can inhibit gram-positive bacteria and be a growth promoter for ruminants. Within the nanchangmycin gene cluster (nan), we identified that two SARP-family regulatory genes, nanR1 and nanR2, were both required to activate the transcription of all nan polyketide genes. Overexpression of NanR1 and NanR2 in wild-type increase NAN yields by at least three folds. Bioinformatic analysis of the immediate upstream DNA sequence of each nan gene and quantitative real-time RT-PCR analysis of the nan operons identified five putative SARP binding sites. Moreover, deletion of an AraC-family repressor gene nanR4 increased expression of NanR1 and R2 and led to a threefold increase in NAN production.

Keywords

SARP Nanchangmycin Streptomyces Regulation 

Supplementary material

203_2011_768_MOESM1_ESM.doc (96 kb)
Supplementary material 1 (DOC 96.0 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.State Key Laboratory of Microbial Metabolism, and School of Life Sciences & BiotechnologyShanghai Jiao Tong UniversityShanghaiChina
  2. 2.School of PharmacyWuhan UniversityWuhanChina

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