Archives of Microbiology

, Volume 194, Issue 5, pp 363–370 | Cite as

Colistin A and colistin B among inhibitory substances of Paenibacillus polymyxa JB05-01-1

  • Karim Naghmouchi
  • Riadh Hammami
  • Ismail Fliss
  • Ron Teather
  • John Baah
  • Djamel Drider
Original Paper

Abstract

Recently, we isolated and reported the antagonism of Paenibacillus polymyxa JB05-01-1 (P. polymyxa JB05-01-1) against Gram-negative bacteria. Here, we provide more insights and attribute the abovementioned antagonism to the production of colistins A and B, which were purified by Amberlite column exchange, C18 column hydrophobicity, superdex 75 16/60 gel filtration chromatography connected to fast protein liquid chromatography and identified by MALDI TOF/TOF, and manual nanospray analysis. The amount of colistin A and colistin B recovered from 500 ml of culture supernatant was about 0.05 mg. The specific activity and the average recovery of the eluted substances were 5,120 AU/mg and 1.1%, respectively. The minimal inhibitory concentrations of the purified colistins against Escherichia coli O157:H7 and Pseudomonas fluorescens LRC R73 were 0.13 and 0.62 μg/ml, respectively.

Keywords

Colistin Paenibacillus polymyxa JB05-01-1 Antagonism Escherichia coli 

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Karim Naghmouchi
    • 1
  • Riadh Hammami
    • 2
  • Ismail Fliss
    • 2
  • Ron Teather
    • 1
  • John Baah
    • 1
  • Djamel Drider
    • 3
  1. 1.Lethbridge Research CenterAgriculture and Agri-Food CanadaLethbridgeCanada
  2. 2.STELA Dairy Research Center, Nutraceuticals and Functional Foods InstituteUniversité LavalQuebecCanada
  3. 3.Laboratoire des Procédés Biologiques, Génie Enzymatique et Microbien (ProBioGEM), UPRES-EA 1026, Polytech’Lille/IUTAUniversité Lille Nord de FranceVilleneuve d’Ascq CedexFrance

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