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Poseidonocella pacifica gen. nov., sp. nov. and Poseidonocella sedimentorum sp. nov., novel alphaproteobacteria from the shallow sandy sediments of the Sea of Japan

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Abstract

The taxonomic study of two Gram-negative, aerobic, non-pigmented bacteria KMM 9010T and KMM 9023T isolated from a sandy sediment sample collected from the Sea of Japan seashore was performed. On the basis of the nearly complete 16S rRNA gene sequences, strains KMM 9010T and KMM 9023T clustered with the Roseobacter lineage (class Alphaproteobacteria) forming a distinct phylogenetic line adjacent to the genus Donghicola. Novel strains shared the highest sequence similarity of 96.4% to each other and lower than 96.1% similarities to other validly named genera of the class Alphaproteobacteria. In both strains, ubiquinone Q-10 was found to be the major respiratory quinone; phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidic acid, and an unknown aminolipid were the major polar lipids and C18:1ω7c and 11-methyl C18:1ω7c were predominant fatty acids. The DNA G+C content was 60.5 mol% (KMM 9010T) and 65.4 mol% (KMM 9023T). Based on phenotypic properties and phylogenetic evidence, strains KMM 9010T and KMM 9023T should be classified as two novel species in a new genus, Poseidonocella gen. nov., with Poseidonocella pacifica sp. nov., the type species with the type strain KMM 9010T (= NRIC 0794T = JCM 17310T), and Poseidonocella sedimentorum sp. nov. as the second species with the type strain KMM 9023T (= NRIC 0796T = JCM 17311T).

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Acknowledgments

The authors thank Dr. Claudine Vereecke, BCCM/LMG Bacteria Collection, Ghent University, Belgium, Dr. Jung-Sook Lee, KCTC, Korean Collection for Type Cultures, Korea, and Dr. Moriya Ohkuma, JCM, Japan Collection of Microorganisms, Japan, for providing type strains. This study was supported by a grant no. 11-04-00781-a from the Russian Foundation for Basic Research (RFBR) and by a grant no. 11-04-98538 from the RFBR and the Far-Eastern Branch of Russian Academy of Sciences.

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Authors and Affiliations

  1. Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, 690022, Vladivostok, Russia

    Lyudmila A. Romanenko & Nataliya I. Kalinovskaya

  2. NODAI Culture Collection Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo, 156-8502, Japan

    Naoto Tanaka

  3. Institute of Marine Biology, Far-Eastern Branch, Russian Academy of Sciences, 690041, Vladivostok, Russia

    Vasily I. Svetashev

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  1. Lyudmila A. Romanenko
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  2. Naoto Tanaka
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  4. Nataliya I. Kalinovskaya
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Correspondence to Lyudmila A. Romanenko.

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Communicated by Erko Stackebrandt.

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Romanenko, L.A., Tanaka, N., Svetashev, V.I. et al. Poseidonocella pacifica gen. nov., sp. nov. and Poseidonocella sedimentorum sp. nov., novel alphaproteobacteria from the shallow sandy sediments of the Sea of Japan. Arch Microbiol 194, 113–121 (2012). https://doi.org/10.1007/s00203-011-0736-3

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  • DOI: https://doi.org/10.1007/s00203-011-0736-3

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