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The in vitro antibiofilm activity of selected marine bacterial culture supernatants against Vibrio spp.

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Abstract

The aim of the work is to investigate the effect of marine bacterial culture supernatants on biofilm formation of Vibrio spp., a major menace in aquaculture industries. Vibrio spp. biofilm cause life-threatening infections in humans and animals. Forty-three marine bacterial culture supernatants were screened against the hydrophobicity index, initial attachment and biofilm formation in Vibrio spp. Twelve culture supernatants showed antibiofilm activity. The bacterial culture supernatants S8-07 (Bacillus pumilus) and S6-01 (B. indicus) inhibited the initial attachment, biofilm formation and dispersed the mature biofilm at 5% v/v concentration without inhibiting the growth. Analysis by light microscopy and confocal laser scanning microscopy showed that the architecture of the biofilm was destroyed by bacterial supernatants when compared to the control. The bacterial supernatants also reduce the surface hydrophobicity of Vibrio spp. which is one of the important requirements for biofilm formation. Further characterization of antibiofilm activity in S8-07 culture supernatant confirmed that it is an enzymatic activity and the size is more than 10 kDa and in S6-01, it is a heat-stable, non-protein compound. Furthermore, both the supernatants failed to show any biosurfactant activity. The culture supernatants of S8-07 and S6-01 with promising antibiofilm property have potential for application in medicine and marine aquaculture.

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Acknowledgments

The authors gratefully acknowledge the computational and bioinformatics facility provided by the Alagappa University Bioinformatics Infrastructure Facility (funded by DBT, GOI; Grant No. BT/BI/25/001/2006). Financial support provided to Chari Nithya by Alagappa University in the form of Research Fellowship is thankfully acknowledged.

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  1. Department of Biotechnology, Alagappa University, Karaikudi, Tamil Nadu, 630 003, India

    Chari Nithya & Shunmugiah Karutha Pandian

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  1. Chari Nithya
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  2. Shunmugiah Karutha Pandian
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Correspondence to Shunmugiah Karutha Pandian.

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Communicated by Jan Roelof van der Meer.

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Nithya, C., Pandian, S.K. The in vitro antibiofilm activity of selected marine bacterial culture supernatants against Vibrio spp.. Arch Microbiol 192, 843–854 (2010). https://doi.org/10.1007/s00203-010-0612-6

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  • DOI: https://doi.org/10.1007/s00203-010-0612-6

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