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Inhibition of uropathogens by lactic acid bacteria isolated from dairy foods and cow’s intestine in western Nigeria

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Abstract

A total of 96 lactic acid bacteria (LAB) were isolated from African indigenous fermented products and cow’s intestines to study their inhibitory capability against multi-drug-resistant uropathogens. Escherichia coli accounted for approximately 45% of isolated uropathogens, followed by Staphylococcus spp. (20%). The Gram negative uropathogens were highly resistant to quinolones, co-trimoxazole, teicoplanin and some β-lactams, while the Staphylococcus spp. showed high resistance to aminoglycosides, β-lactams and macrolides. Twenty-four LAB isolates were selected based on their antimicrobial activity against two uropathogenic Staphylococcus aureus strains and bacteriocin production. LAB strains showing antimicrobial activity were grouped into smaller groups through amplified ribosomal DNA restriction analysis (ARDRA). Representative strains were identified as Weissella spp., Enterococcus faecium, Lactococcus lactis and Lactobacillus brevis through sequencing of 16S rDNA. The Weissella spp. and L. brevis strains demonstrated remarkable inhibitory activity against seven strains of Gram negative uropathogens. Two strains of L. lactis produced a bacteriocin-like inhibitory substance active against Lactobacillus sakei. In this study, an unusual high rate of co-trimoxazole, quinolones and macrolides resistance among uropathogens from south west Nigeria was discovered. Based on their sensitivity to Weissella spp., there is a potential for using these LAB as a natural approach for the protection against the uropathogens assayed.

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Acknowledgments

We are grateful to the staffs of medical microbiology units of University College Hospital, Ibadan, Nigeria and State Specialist Hospital, Ado Ekiti, Nigeria for their assistance in isolates collection. F.A. Ayeni wishes to acknowledge the EMBO for the short-term fellowship grant during which some part of this work was done. Part of this work was supported by the Spanish Ministerio de Ciencia e Innovación (grants: AGL2004-07285-C02-01 and Consolider FUN-C-FOD CSD2007-063) and Comunidad Autónoma de Madrid (ALIBIRD: S-0505/AGR-0153).

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Authors and Affiliations

  1. Department of Pharmaceutical Microbiology, University of Ibadan, Ibadan, Nigeria

    Funmilola A. Ayeni & Bolanle A. Adeniyi

  2. Department of Botany and Microbiology, University of Ibadan, Ibadan, Nigeria

    Samuel T. Ogunbanwo

  3. Department of Dairy Science and Technology, Instituto del Frío (CSIC), Madrid, Spain

    Raquel Tabasco, Torsten Paarup & Carmen Peláez

  4. Department of Dairy Science and Technology, Instituto del Frío (CSIC), Jose Antonio Novais 10, 28040, Madrid, Spain

    Teresa Requena

Authors
  1. Funmilola A. Ayeni
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  2. Bolanle A. Adeniyi
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  3. Samuel T. Ogunbanwo
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  4. Raquel Tabasco
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  5. Torsten Paarup
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  6. Carmen Peláez
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  7. Teresa Requena
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Corresponding author

Correspondence to Teresa Requena.

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Communicated by Erko Stackebrandt.

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Ayeni, F.A., Adeniyi, B.A., Ogunbanwo, S.T. et al. Inhibition of uropathogens by lactic acid bacteria isolated from dairy foods and cow’s intestine in western Nigeria. Arch Microbiol 191, 639–648 (2009). https://doi.org/10.1007/s00203-009-0492-9

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  • DOI: https://doi.org/10.1007/s00203-009-0492-9

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