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Involvement of digalactosyldiacylglycerol in cellular thermotolerance in Synechocystis sp. PCC 6803

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Abstract

The effects of digalactosyldiacylglycerol (DGDG) deficiency on photosynthesis at high temperatures were examined using a dgdA mutant of Synechocystis sp. PCC 6803 incapable of DGDG biosynthesis. The dgdA mutant cells showed significant growth retardation when the temperature was increased from 30 to 38°C, although wild-type cells grew normally. The degree of growth retardation was enhanced by increasing light intensity. In addition, dgdA mutant cells showed increased sensitivity to the photoinhibition of photosynthesis when illuminated at 38°C. Analysis of photosynthesis in intact cells suggested that the inhibition of repair processes and accelerated photodamage resulted in growth retardation in dgdA mutant cells at high temperatures.

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Acknowledgments

This work was supported by Grants-in-Aid for Scientific Research (no. 20608002 to N. M., no. 20017006 to N. S., and no. 20570031 to H. W.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan

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Authors and Affiliations

  1. Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo, 153-8902, Japan

    Naoki Mizusawa, Shinya Sakata, Isamu Sakurai, Naoki Sato & Hajime Wada

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  1. Naoki Mizusawa
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  2. Shinya Sakata
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  3. Isamu Sakurai
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  4. Naoki Sato
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  5. Hajime Wada
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Correspondence to Hajime Wada.

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Communicated by Jack Meeks.

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Mizusawa, N., Sakata, S., Sakurai, I. et al. Involvement of digalactosyldiacylglycerol in cellular thermotolerance in Synechocystis sp. PCC 6803. Arch Microbiol 191, 595–601 (2009). https://doi.org/10.1007/s00203-009-0486-7

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  • DOI: https://doi.org/10.1007/s00203-009-0486-7

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