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Enzymatic, outer membrane proteins and plasmid alterations of starved Vibrio parahaemolyticus and Vibrio alginolyticus cells in seawater

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Abstract

The marine bacteria Vibrio parahaemolyticus and V. alginolyticus were incubated in seawater for 8 months to evaluate their adaptative responses to starvation. The starved cells showed an altered biochemical and enzymatic profiles, respectively, on Api 20E and Api ZYM systems and an evolution to the filterable minicells state capable to pass membrane pore size 0.45 μm. Outer membrane proteins patterns of stressed bacteria were also altered. Indeed, these modifications were manifested by the appearance and/or disappearance of bands as well as in the level of expression of certain proteins. Plasmids profiles analysis showed that V. alginolyticus ATCC 33787 lost three plasmids, whereas other tested strains conserved their initial profiles.

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Acknowledgments

We are grateful to M’SELMI Abdelhamid principal technical; USCR: Unité des Services Communs de Recherche, Faculté des Sciences de Tunis. Campus Universitaire. Tunisia.

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Authors and Affiliations

  1. Laboratoire d’Analyse, Traitement et Valorisation des Polluants de l’Environnement et des Produits, Faculté de Pharmacie Rue Avicenne, 5000, Monastir, Tunisia

    Fethi Ben Abdallah & Amina Bakhrouf

  2. Unité de Fermentation et de Développement de Vaccins Virologiques, Institut Pasteur de Tunis, 13 Place Pasteur, 1002, Tunis, Tunisia

    Fethi Ben Abdallah & Héla Kallel

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  1. Fethi Ben Abdallah
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  2. Héla Kallel
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  3. Amina Bakhrouf
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Correspondence to Fethi Ben Abdallah.

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Communicated by Jorge Membrillo-Hernández.

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Abdallah, F.B., Kallel, H. & Bakhrouf, A. Enzymatic, outer membrane proteins and plasmid alterations of starved Vibrio parahaemolyticus and Vibrio alginolyticus cells in seawater. Arch Microbiol 191, 493–500 (2009). https://doi.org/10.1007/s00203-009-0477-8

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  • DOI: https://doi.org/10.1007/s00203-009-0477-8

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