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Physico-chemical characterization of a new heteropolysaccharide produced by a native isolate of heterofermentative Lactobacillus sp. CFR-2182

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Abstract

A heterofermentative Lactobacillus sp. CFR-2182 was isolated from dahi samples and it was found to produce 8.0 and 20.5 g/L heteropolysaccharide (HePS) in EPS medium (a simplified synthetic medium) and modified MRS broth, respectively, after 72 h at 30°C. The total carbohydrate, reducing sugar and moisture contents of the purified HePS were 74, 10.6 and 2 g, respectively, per 100 g on dry weight basis. The HePS produced in EPS medium had glucose and mannose in 17:1 ratio. The HePS was non-gelling and non-film forming type. It was completely soluble in water and 1 N sodium hydroxide solution. Gel permeation chromatography and HPLC analysis indicated considerable heterogeneity of the HePS, having three fractions with molecular weights ranging from 3.3 × 104 to 1.32 × 106 Da. The enzymatic hydrolysis of the HePS with pullulanase and α-amylase [with α(1→4) linkage] indicated the presence of α(1→6) and traces of α(1→4) linkages, respectively. NMR analysis of the EPS revealed unique chemical shifts.

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Acknowledgments

The research grant received from United Nations University, Tokyo, as a follow-up project of UNU-Kirin Fellowship to first author is highly acknowledged. Authors are thankful to Director, CFTRI, and Mysore for providing the facilities. The technical help received from the staff at Central Instrumentation Services & Facility, and NMR facility, CFTRI, Mysore, in analyzing the polymer is acknowledged.

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Authors and Affiliations

  1. Food Microbiology Department, Central Food Technological Research Institute, Mysore, 570020, India

    S. V. N. Vijayendra & G. Palanivel

  2. Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, 570020, India

    S. Mahadevamma & R. N. Tharanathan

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  1. S. V. N. Vijayendra
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  2. G. Palanivel
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  3. S. Mahadevamma
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  4. R. N. Tharanathan
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Correspondence to S. V. N. Vijayendra.

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Communicated by Erko Stackebrandt.

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Vijayendra, S.V.N., Palanivel, G., Mahadevamma, S. et al. Physico-chemical characterization of a new heteropolysaccharide produced by a native isolate of heterofermentative Lactobacillus sp. CFR-2182. Arch Microbiol 191, 303–310 (2009). https://doi.org/10.1007/s00203-008-0453-8

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