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Archives of Microbiology

, 190:333 | Cite as

Regulation of gvp genes encoding gas vesicle proteins in halophilic Archaea

  • Sandra Scheuch
  • Larissa Marschaus
  • Simone Sartorius-Neef
  • Felicitas Pfeifer
Original Paper

Abstract

Three gas vesicle gene clusters derived from Halobacterium salinarum (p-vac and c-vac) and Haloferax mediterranei (mc-vac) are used as model systems to study gene regulation in Archaea. An unusual pair of regulatory proteins is involved here, with GvpE acting as transcription activator and GvpD exhibiting a repressing function. Both regulators are able to interact leading to the loss of GvpE and the repression (or turnoff) of the gas vesicle formation. The latter function of GvpD requires a p-loop motif and an arginine-rich region, bR1. Both regulator proteins are differentially expressed from the same gvp transcript in Hfx. mediterranei and Hbt. salinarum PHH4. GvpE appears to recognize a 20-nucleotide activator sequence (UAS) located upstream and adjacent to the TFB-recognition element BRE of the two promoters driving the transcription of the divergently oriented gvpACNO and gvpDEFGHIJKLM gene clusters. The BRE elements of these two promoters are separated by 35 nucleotides only, and the distal portions of the two GvpE-UAS overlap considerably in the center of this region. Mutations here negatively affect the GvpE-induced activities of both gvp promoters, whereas alterations in the proximal UAS portions only affect the activity of the promoter located close by.

Keywords

GvpE activator TFB and TBP Gas vesicle genes 

Abbreviations

UAS

Upstream activator sequence

TBP

TATA-box binding protein

TFB

Transcription factor B

RNAP

DNA-dependent RNA polymerase

Notes

Acknowledgments

This work was financially supported by the Deutsche Forschungsgemeinschaft (DFG) as part of the priority programme SPP 1112 “Genome function and gene regulation in Archaea” (Pf 165/8-5). Reinhard Mentele and Friedrich Lottspeich (MPI für Biochemie, Martinsried, Germany) are thanked for the N-terminal sequence determination of PitA, and Katharina Teufel for critical reading of the manuscript.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Sandra Scheuch
    • 1
  • Larissa Marschaus
    • 1
  • Simone Sartorius-Neef
    • 1
  • Felicitas Pfeifer
    • 1
  1. 1.Institut für Mikrobiologie und GenetikTechnische Universität DarmstadtDarmstadtGermany

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