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Characterization of a new organization of the plantaricin locus in the inducible bacteriocin-producing Lactobacillus plantarum J23 of grape must origin

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Abstract

Lactobacillus plantarum J23 was previously characterized as a bacteriocin-producer-strain when it was cocultured with other lactic acid bacteria. In this work, the genetic organization of the pln locus in the J23 strain was studied and compared with those of previously described L. plantarum C11, WCFS1 and NC8 strains. A new organization of the plantaricin locus was detected in the J23 strain. The sequenced fragment (20,266 bp) comprised plnJLR, plnMNOP, plnEFI, plnGHSTUVWXY, and plNC8IF-plNC8HK-plnD operons, as well as a new region that includes three new orfs (GenBank accession number DQ323671). When the J23 pln gene sequences were compared with those included in the GenBank database, the identity of the putative encoded proteins was in the range 67.1–100%. The regulatory system and the repertoire of putative bacteriocins of the J23 pln locus presented important differences with respect to the ones of C11, WCFS1 and NC8, such as the absence of plnK and the presence of a larger plnJ gene than the previously described for the other L. plantarum strains. The pln locus in L. plantarum strains seems to be a mosaic-like structure with different modules and reorganizations that presents highly conserved regions related to transport and bacteriocin maturation and variable regions related to regulation and bacteriocin production.

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Acknowledgments

This work was supported in part by a grant (AGL2002-2293) from the Ministry of Education and Science of Spain and FEDER of the European Community and from the Gobierno de La Rioja (ACPI2003/01). B. Rojo-Bezares was supported by a doctorate grant from the Spanish Ministry of Education and Science (BI-UR-03/16586.840) and Y. Sáenz is a contractual technician supported by grant PTA-2003-02-00002 from the Spanish Ministry of Education and Science.

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Authors and Affiliations

  1. Área de Bioquímica y Biología Molecular, Departamento de Agricultura y Alimentación, Universidad de La Rioja, Madre de Dios 51, 26006, Logroño, Spain

    Beatriz Rojo-Bezares, Yolanda Sáenz, Laura Navarro, Myriam Zarazaga, Fernanda Ruiz-Larrea & Carmen Torres

  2. Departamento de Biotecnología, Instituto de la Grasa, CSIC, Sevilla, Spain

    Rufino Jiménez-Díaz

Authors
  1. Beatriz Rojo-Bezares
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  2. Yolanda Sáenz
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  3. Laura Navarro
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  4. Rufino Jiménez-Díaz
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  5. Myriam Zarazaga
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  6. Fernanda Ruiz-Larrea
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  7. Carmen Torres
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Corresponding author

Correspondence to Carmen Torres.

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Communicated by Erko Stackebrandt.

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Rojo-Bezares, B., Sáenz, Y., Navarro, L. et al. Characterization of a new organization of the plantaricin locus in the inducible bacteriocin-producing Lactobacillus plantarum J23 of grape must origin. Arch Microbiol 189, 491–499 (2008). https://doi.org/10.1007/s00203-007-0342-6

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  • DOI: https://doi.org/10.1007/s00203-007-0342-6

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