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Structural analysis of cell wall mannan of Candida sojae, a new yeast species isolated from defatted soybean flakes

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Abstract

We investigated the structural and immunochemical characteristics of cell wall mannan obtained from Candida sojae JCM 1644, which is a new yeast species isolated from defatted soybean flakes. The results of a slide-agglutination test and of an enzyme-linked immunosorbent assay using anti-factor sera to the pathogenic Candida species indicated that the cells and the C. sojae mannan were cross-reactive to the specific anti-factor sera against Candida albicans serotype A (FAb 6) and Candida guilliermondii (FAb 9). Two-dimensional homonuclear Hartmann–Hahn analysis indicated that the mannan consisted of various linked oligomannosyl side chains containing α-1,2-, α-1,3-, α-1,6- and β-1,2-linked mannose residues. However, although the determinants of antigenic factors 6 and 9 could be not found in this mannan, branched side chains, Manβ1-2Manα1-3[Manα1-6]Manα1-(2Manα1-)n2Man and a linear α-1,6-linked polymannosyl backbone, which are cross-reacted by FAbs 6 and 9, respectively, were identified. The mannan was subjected to acetolysis in order to determine the polymerization length of the α-1,2-linked oligomannosyl residue in the side chains. The result of 1H-nuclear magnetic resonance analysis of the released oligosaccharides showed that the remarkable regularity in the length of α-1,2-linked oligomannosyl side chains, which were previously found in mannans of other Candida species, is not observed in this mannan.

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Abbreviations

ELISA:

Enzyme-linked immunosorbent assay

NMR:

Nuclear magnetic resonance

2D-HOHAHA:

Two-dimensional homonuclear Hartmann–Hahn

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Authors and Affiliations

  1. Department of Microbiology, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki, 859-3298, Japan

    Hiroko Oyamada, Yukiko Ogawa & Hidemitsu Kobayashi

  2. Department of Infection and Host Defense, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi, 981-8558, Japan

    Nobuyuki Shibata & Yoshio Okawa

  3. Sendai Research Institute for Mycology, 1-14-34 Tohshogu, Aoba-ku, Sendai, Miyagi, 981-0908, Japan

    Shigeo Suzuki

Authors
  1. Hiroko Oyamada
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  2. Yukiko Ogawa
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  3. Nobuyuki Shibata
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  4. Yoshio Okawa
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  5. Shigeo Suzuki
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  6. Hidemitsu Kobayashi
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Correspondence to Hidemitsu Kobayashi.

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Communicated by Erko Stackebrandt.

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Oyamada, H., Ogawa, Y., Shibata, N. et al. Structural analysis of cell wall mannan of Candida sojae, a new yeast species isolated from defatted soybean flakes. Arch Microbiol 189, 483–490 (2008). https://doi.org/10.1007/s00203-007-0339-1

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  • DOI: https://doi.org/10.1007/s00203-007-0339-1

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