Skip to main content
SpringerLink
Log in

Characteristics of cell-mediated, anti-listerial immunity induced by a naturally avirulent Listeria monocytogenes serotype 4a strain HCC23

  • Original Paper
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

The characteristics of cell-mediated, anti-listerial immune response initiated by an avirulent Listeria monocytogenes serotype 4a strain HCC23 was assessed. Similar to virulent strain EGD, avirulent strain HCC23 grew readily within macrophage-like J774 cells, but nonhemolytic strain ATCC 15313 did not. Compared with EGD, HCC23 induced a relatively low level of gamma interferon (IFN-γ) in mice, and ATCC 15313 stimulated no detectable IFN-γ. The percentages of gated CD4 T cells from mice immunized with EGD and HCC23 showed a notable drop (to 30%) at 21 days post exposure in comparison with that (about 50%) from ATCC 15313-injected or untreated mice; and the percentage of gated NK cells from EGD-immunized group was markedly higher than those from other treatment groups. Mice immunized with HCC23 and EGD developed an equally strong protective immunity against listeriosis that was effective in both short and long terms, but those injected with ATCC 15313 or saline succumbed to listeriosis within 6 days of challenge.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Badovinac VP, Harty JT (2000) Adaptive immunity and enhanced CD8+ T cell response to Listeria monocytogenes in the absence of perforin and IFN-gamma. J Immunol 164:6444–6452

    PubMed  CAS  Google Scholar 

  • Baldridge JR, Thomashow MF, Hinrichs DJ (1988) Induction of immunity with avirulent Listeria monocytogenes 19113 depends on bacterial replication. Infect Immun 56:2109–2113

    PubMed  CAS  Google Scholar 

  • Chakraborty T, Ebel F, Wehland J, Dufrenne J, Notermans S (1994) Naturally occurring virulence-attenuated isolates of Listeria monocytogenes capable of inducing long term protection against infection by virulent strains of homologous and heterologous serotypes. FEMS Immunol Med Microbiol 10:1–9

    Article  PubMed  CAS  Google Scholar 

  • Erdenlig S, Ainsworth AJ, Austin FW (2000) Pathogenicity and production of virulence factors by Listeria monocytogenes isolates from channel catfish. J Food Prot 63:613–619

    PubMed  CAS  Google Scholar 

  • Harty JT, Bevan MJ (1995) Specific immunity to Listeria monocytogenes in the absence of IFN gamma. Immunity 3:109–117

    Article  PubMed  CAS  Google Scholar 

  • Huang S, Hendriks W, Althage A, Hemmi S, Bluethmann H, Kamijo R, Vilcek J, Zinkernagel RM, Aguet M (1993) Immune response in mice that lack the interferon-gamma receptor. Science 259:1742–1745

    Article  PubMed  CAS  Google Scholar 

  • Kaufmann SH (1984) Acquired resistance to facultative intracellular bacteria: relationship between persistence, cross-reactivity at the T-cell level, and capacity to stimulate cellular immunity of different Listeria strains. Infect Immun 45:34–41

    Google Scholar 

  • Kaufmann SH (1993) Immunity to intracellular bacteria. Annu Rev Immunol 11:129–163

    Article  PubMed  CAS  Google Scholar 

  • Liu D (2004) Listeria monocytogenes: comparative interpretation of mouse virulence assay. FEMS Microbiol Lett 233:159–164

    Article  PubMed  CAS  Google Scholar 

  • Liu D, Ainsworth AJ, Austin FW, Lawrence ML (2003) Characterization of virulent and avirulent Listeria monocytogenes strains by PCR amplification of putative transcriptional regulator and internalin genes. J Med Microbiol 52:1066–1070

    Article  CAS  Google Scholar 

  • Liu D, Lawrence ML, Austin FW, Ainsworth AJ (2005) Comparative assessment of acid, alkali and salt tolerance in Listeria monocytogenes virulent and avirulent strains. FEMS Microbiol Lett 243:373–378

    Article  PubMed  CAS  Google Scholar 

  • Liu D, Lawrence ML, Gorski L, Mandrell RE, Austin FW, Ainsworth AJ (2006) Listeria monocytogenes serotype 4b strains belonging to lineages I and III possess distinct molecular features. J Clin Microbiol 44:214–217

    Article  PubMed  CAS  Google Scholar 

  • Pamer EG (2004) Immune responses to Listeria monocytogenes. Nat Rev Immunol 4:812–823

    Article  PubMed  CAS  Google Scholar 

  • Vazquez-Boland JA, Kuhn M, Berche P, Chakraborty T, Dominguez-Bernal G, Goebel W, Gonzalez-Zorn B, Wehlan J, Kreft J (2001) Listeria pathogenesis and molecular virulence determinants. Clin Microbiol Rev 14:584–640

    Article  PubMed  CAS  Google Scholar 

  • Von Koenig CH, Heymer B, Hof H, Finger H (1983) Course of infection and development of immunity in experimental infection of mice with Listeria serotypes. Infect Immun 40:1170–1177

    Google Scholar 

  • Xiong H, Tanabe Y, Ohya S, Mitsuyama M (1998) Administration of killed bacteria together with listeriolysin O induces protective immunity against Listeria monocytogenes in mice. Immunology 94:14–21

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by US Department of Agriculture, Agricultural Research Service (Agreement No. 58-6202-5-083).

Author information

Authors and Affiliations

  1. College of Veterinary Medicine, Mississippi State University, P.O. Box 6100, Mississippi State, MS, 39762, USA

    Dongyou Liu, Mark L. Lawrence, Lesya M. Pinchuk, A. Jerald Ainsworth & Frank W. Austin

Authors
  1. Dongyou Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mark L. Lawrence
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lesya M. Pinchuk
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Jerald Ainsworth
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Frank W. Austin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dongyou Liu.

Additional information

Communicated by Erko Stackebrandt.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, D., Lawrence, M., Pinchuk, L.M. et al. Characteristics of cell-mediated, anti-listerial immunity induced by a naturally avirulent Listeria monocytogenes serotype 4a strain HCC23. Arch Microbiol 188, 251–256 (2007). https://doi.org/10.1007/s00203-007-0242-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00203-007-0242-9

Keywords

Search

Navigation