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Genetic diversity of phenazine- and pyoluteorin-producing pseudomonads isolated from green pepper rhizosphere

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Abstract

The genetic diversity among indigenous phenazine-1-carboxylic acid (PCA)-producing and pyoluteorin (Plt)-producing isolates of pseudomonads screened from green pepper rhizosphere was exploited in this study. A total of 48 bacterium isolates producing one or both of these antibiotics were screened from green pepper rhizosphere in diverse regions in China. Among these isolates, 45 could produce PCA, 3 could produce both PCA and Plt, and none could produce Plt only. Based on the restriction patterns of partial 16S and 16S-23S internal transcribed spacer (ITS) PCR fragments generated by enzyme HaeIII or HinfI, these isolates fell into 19 or 17 distinct groups respectively, indicating that there was a significant diversity among them. Polygenetic analysis of the partial 16S rDNA and 16S-23S ITS sequence from the representative in each group in the context of similar sequence from previously described bacterial species indicated that most isolates were closely related to the species of Pseudomonas fluorescens, P. putida, and Stenotrophomonas maltophilia. Some of these representatives of these isolates, then, are likely to be novel strains or species in these two genera.

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Acknowledgments

Dr. Linda S. Thomashow is thanked for her valuable suggestions of this manuscript. This work was financially supported both by Grant No. 2004BA308A02–14 from the Tenth Five-Year Programs of Chinese National Science and Technology Development and Grant No. 30370041 from the National Science Foundation of China.

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Authors and Affiliations

  1. College of Life Science and Biotechnology, Shanghai Jiaotong University, 800 Dongchuan RD, 200240, Shanghai, China

    Haiming Liu, Dexian Dong, Huasong Peng, Xuehong Zhang & Yuquan Xu

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  1. Haiming Liu
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  2. Dexian Dong
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  3. Huasong Peng
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  4. Xuehong Zhang
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  5. Yuquan Xu
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Corresponding author

Correspondence to Yuquan Xu.

Additional information

The GenBank accession numbers for DNA sequences of the partial 16S rDNA with ITS region in each isolate determined in this study were: GP30 DQ003219; GP127 DQ003220; GP83 DQ003221; GP42, DQ003222; GP59 DQ003223; GP50 DQ003224; GP36 DQ003225; GP110 DQ003226; GP26 DQ003227; GP37 DQ003228; GP60 DQ003229; GP31 DQ003230; GP57 DQ003231; GP75 DQ003232; GP115 DQ003233; GP65 DQ003234; GP32 DQ003235; GP76 DQ003236; GP78 DQ003237.

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Liu, H., Dong, D., Peng, H. et al. Genetic diversity of phenazine- and pyoluteorin-producing pseudomonads isolated from green pepper rhizosphere. Arch Microbiol 185, 91–98 (2006). https://doi.org/10.1007/s00203-005-0072-6

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  • DOI: https://doi.org/10.1007/s00203-005-0072-6

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