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Production and partial characterization of extracellular proteinases from Streptomyces malaysiensis, isolated from a Brazilian cerrado soil

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Abstract

Streptomyces malaysiensis AMT-3, isolated from a Brazilian cerrado soil, showed proteolytic activities detected by gelatin–sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The optimum proteinase production was obtained when using 2.5% wheat bran and 0.1% yeast extract in the culture medium, after 5 days incubation at 30°C. The enzymatic complex degraded gelatin optimally at pH 7.0, and under these conditions eight proteolytic bands (four serine-proteinases and four metaloproteinases), ranging from 20 to 212 kDa, were detected on the culture supernatant filtrates. In addition, a 35-kDa proteinase was thermostable at 60°C for 120 min. These results point out to the applicability of gelatin zymograms in the characterization of crude enzymatic complexes. According to our results, this enzymatic complex could be used for biotechnological applications.

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Acknowledgements

This work was supported by Fundação Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ), Centro Nacional de Desenvolvimento Científico e Tecnológico (MCT/CNPq), Programa de Apoio a Núcleos de Excelência (PRONEX), Financiadora de Estudos e Projetos – Banco Interamericano de Desenvolvimento (FINEP-BID), Coordenação de Aperfeiçoamento de Pessoal do Ensino Superior (CAPES) and Conselho de Ensino para Graduados e Pesquisas (CEPG/UFRJ).

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Authors and Affiliations

  1. Dep. Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes, CCS, Universidade Federal do Rio de Janeiro (UFRJ), CEP, 21941-590, Rio de Janeiro, Brazil

    Rodrigo P. Nascimento, Claudia M. d’Avila-Levy, Rodrigo F. Souza, Marta H. Branquinha & Rosalie R. R. Coelho

  2. Dep. Bioquímica, Instituto de Química, CT, UFRJ, Brazil

    Elba P. S. Bon

  3. Escola de Química, CT, UFRJ, Brazil

    Nei Pereira-Jr

Authors
  1. Rodrigo P. Nascimento
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  2. Claudia M. d’Avila-Levy
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  3. Rodrigo F. Souza
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  4. Marta H. Branquinha
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  5. Elba P. S. Bon
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  6. Nei Pereira-Jr
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  7. Rosalie R. R. Coelho
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Correspondence to Rodrigo P. Nascimento.

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Nascimento, R.P., d’Avila-Levy, C.M., Souza, R.F. et al. Production and partial characterization of extracellular proteinases from Streptomyces malaysiensis, isolated from a Brazilian cerrado soil. Arch Microbiol 184, 194–198 (2005). https://doi.org/10.1007/s00203-005-0030-3

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  • DOI: https://doi.org/10.1007/s00203-005-0030-3

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