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Effect of glutathione on UV induction of prophage lambda

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Abstract

The effect of glutathione (GSH) on the ultraviolet (UV) induction of lambda prophage was investigated in lysogenic Escherichia coli. The data showed that extracellular GSH could inhibit the UV induction of lambda prophage. The inhibitory rates were concentration dependent, and the maximal rate obtained was 94% with 3.0 M GSH. The effect was also measured in three different lambda lysogens: a wild-type strain (wt), an isogenic GSH-deficient strain, and an isogenic strain producing increased amounts of GSH. The result showed that when subjected to UV irradiation (254 nm, 60 J m−2), GSH-deficient strain was approximately fivefold more sensitive to be lysed than wt, whereas the strain with higher intracellular GSH levels was only 28% susceptible to be lysed. With electron spin resonance and spin trapping techniques, we observed that free radical signals occurred in the suspensions of UV irradiated lysogenic cells and the intensity of signals was influenced by GSH levels. These results indicate that GSH can significantly inhibit the UV induction of lambda prophage, and that this effect is correlated to its capacity to scavenge free radicals generated after UV irradiation.

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Acknowledgements

We thank Dr. Barry L. Wanner for providing pKD46 and pKD3. We are also grateful to Dr. Qian Chen from the Technical Institute of Physics and Chemistry of CAS for ESR detection, and PhD. Jeff Clogston from the Ohio State University for his assistance during the revision of this paper.

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Authors and Affiliations

  1. College of Life Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Yancheng Liu, Qiuju Zhang, Chengxiang Fang, Shiqiao Zhu, Yali Tang & Shaoyi Huang

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  1. Yancheng Liu
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  2. Qiuju Zhang
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  3. Chengxiang Fang
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  4. Shiqiao Zhu
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Correspondence to Chengxiang Fang.

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Liu, Y., Zhang, Q., Fang, C. et al. Effect of glutathione on UV induction of prophage lambda. Arch Microbiol 183, 444–449 (2005). https://doi.org/10.1007/s00203-005-0019-y

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  • DOI: https://doi.org/10.1007/s00203-005-0019-y

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