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Identification of IS elements in Acidithiobacillus ferrooxidans strains grown in a medium with ferrous iron or adapted to elemental sulfur

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Abstract

IS elements were identified in the genomes of five Acidithiobacillus ferrooxidans strains isolated from various media. IST2 elements were revealed in all the strains grown in a medium with ferrous iron, ISAfe1 elements were detected in four strains (TFBk, TFL-2, TFV-1 and TFO). Three strains (TFV-1, TFN-d and TFO) were found to contain IS elements, ~600 bp long. These were named preliminary as ISAfe600. Partial sequencing of the 5′- and 3′-terminal nucleotide stretches of an ISAfe1 element in TFBk and TFL-2 strains and complete sequencing of the ISAfe1 element in the TFBk strain has revealed nucleotide substitutions as compared to the prototype, i.e., the ISAfe1 element of an ATCC 19859 strain. Partial sequencing of the 5′- and 3′-terminal nucleotide stretches of the IST2 elements in TFO, TFBk and TFL-2 strains has shown numerous nucleotide substitutions when compared to the IST2 element of an ATCC 19859 strain. Complete sequencing of the IST2 element in the TFBk strain has revealed: the divergence between the IST2 elements in the TFBk strain and the prototype was 21.2%. Southern hybridization of EcoRI fragments of the chromosomal DNA from five A. ferrooxidans strains grown in a medium with ferrous iron using an internal region of ISAfe1, a full-length ISAfe1 or a full-length IST2 as probes has shown them to differ in the number of copies of IS elements and their localization on the chromosomes. Adaptation to elemental sulfur in A. ferrooxidans strains caused changes in the number, intensity and localization of hybridization bands. The authors discuss the role of IS elements in the adaptation of A. ferrooxidans to the new energy substrate.

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Acknowledgements

This research was carried out with the support of the following grants: “Leading Scientific Schools” No. 1742.2003.4; Initiative Scientific Projects RFFI No. 01-04-48270 and No. 03-04-49294; and State Contract No. 43.073.1.1.2515.

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Authors and Affiliations

  1. Laboratory of chemolithotrophic microorganisms, Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let. Oktyabrya 7/2, Moscow, 117312, Russia

    Tamara F. Kondrat’eva, Svetlana N. Ageeva & Grigory I. Karavaiko

  2. Laboratory of neuroreceptors and neuroregulators, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miclukho-Maklaya 16/10, Moscow, 117871, Russia

    Vasily N. Danilevich

Authors
  1. Tamara F. Kondrat’eva
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  2. Vasily N. Danilevich
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  3. Svetlana N. Ageeva
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  4. Grigory I. Karavaiko
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Corresponding author

Correspondence to Tamara F. Kondrat’eva.

Additional information

The nucleotide sequence data reported in this paper were deposited in GenBank under accession numbers: AY823401, the ISAfe1 from A. ferrooxidans TFBk; AY825254, the IST2 from TFBk; DQ002894, the 5′-terminal nucleotide sequence of ISAfe1 from TFL-2; DQ002895, the 3′-terminal nucleotide sequence of ISAfe1 from TFL-2; DQ005952, the 5′-terminal nucleotide sequence of IST2 from TFV-1; DQ005953, the 3′-terminal nucleotide sequence of IST2 from TFV-1.

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Kondrat’eva, T.F., Danilevich, V.N., Ageeva, S.N. et al. Identification of IS elements in Acidithiobacillus ferrooxidans strains grown in a medium with ferrous iron or adapted to elemental sulfur. Arch Microbiol 183, 401–410 (2005). https://doi.org/10.1007/s00203-005-0010-7

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  • DOI: https://doi.org/10.1007/s00203-005-0010-7

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