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Relations and functions of dye-linked formaldehyde dehydrogenase from Hyphomicrobium zavarzinii revealed by sequence determination and analysis

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Abstract

faoA, the gene of the dye-linked NAD(P)-independent quinone-containing formaldehyde dehydrogenase of methylamine-grown Hyphomicrobium zavarzinii strain ZV 580 was sequenced and analyzed together with an apparent promoter region and adjoining genes in a 7.2-kb fragment of hyphomicrobial DNA. The formaldehyde dehydrogenase, identified as a periplasmic enzyme by its signal sequence, is distantly related to the soluble pyrroloquinoline-quinone-dependent glucose dehydrogenase of Acinetobacter calcoaceticus and to other predicted glucose dehydrogenase sequences. The promoter region, containing about 400 nucleotides upstream of faoA, comprised potential binding sites identical or highly similar to known consensus sequences of the sigma factors σ70 (housekeeping), σH (heat shock), σF (flagellar) and σN (nitrogen). The complex regulation of the transcription of faoA, which is suggested by this setting and emphasized by a possible heat-shock promoter, supports a hypothesis proposing an auxiliary role of the enzyme in lowering detrimental elevated concentrations of formaldehyde, which might arise in the course of stress or regulatory transitions disturbing balanced C1 metabolism.

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Abbreviations

Ac-sGDH:

Soluble glucose dehydrogenase from Acinetobacter calcoaceticus

FalDH:

Dye-linked formaldehyde dehydrogenase

GDH:

Glucose dehydrogenase

H4MPT:

Tetrahydromethanopterin

PQQ:

Pyrroloquinoline quinone

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Acknowledgements

The expert technical assistance of Mrs Dagmar Wenzel in maintaining a functional lab is gratefully acknowledged by A.C.S. This work was supported by Deutsche Forschungsgemeinschaft (grants Schw 163/9-1 and Me 765/8-1).

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Author notes

  1. Bernd Moritz

    Present address: F. Hoffmann-La Roche Pharmaceuticals Division, Grenzacher Str., 4070, Basel, Switzerland

Authors and Affiliations

  1. Institut für Molekulare Physiologie und Biotechnologie der Pflanzen (IMBIO), Universität Bonn, Kirschallee 1, 53115, Bonn, Germany

    Arnold C. Schwartz & Gaby Gockel

  2. Medizinisches Proteom-Center der Ruhr-Universität Bochum, Universitätsstr. 150, 44780, Bochum, Germany

    Julia Groß, Bernd Moritz & Helmut E. Meyer

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  1. Arnold C. Schwartz
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  2. Gaby Gockel
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Correspondence to Arnold C. Schwartz.

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This paper is dedicated to Prof. Dr H. G. Schlegel on the occasion of his 80th birthday.

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Schwartz, A.C., Gockel, G., Groß, J. et al. Relations and functions of dye-linked formaldehyde dehydrogenase from Hyphomicrobium zavarzinii revealed by sequence determination and analysis. Arch Microbiol 182, 458–466 (2004). https://doi.org/10.1007/s00203-004-0730-0

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