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Phylogenetic analysis of Verticillium species based on nuclear and mitochondrial sequences

Archives of Microbiology volume 181, pages 435–442 (2004)Cite this article

Abstract

Three different genes were sequenced from isolates of five plant-pathogenic Verticillium species, Verticillium albo-atrum, Verticillium dahliae, Verticillium longisporum, Verticillium nigrescens, and Verticillium tricorpus. The sequences covered parts of the mitochondrial cytochrome b gene (cob), the mitochondrial small subunit rRNA gene (rns) and the nuclear ITS2 region. When the sequences were combined, the five species clustered in five monophyletic groups, with V. nigrescens distantly related to the other species while V. tricorpus displayed a somewhat closer relationship to the three remaining species. V. albo-atrum, V. dahliae and V. longisporum were found to be very similar to each other, with V. albo-atrum and V. longisporum displaying the closest relationship. The species affiliation of V. longisporum is discussed.

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Acknowledgements

We thank Gunilla Swärdh, Ingrid Schenning and Yvonne Tillman for providing skilful technical assistance. Prof. Ulf Lagercrantz and Dr Jens Sundström are acknowledged for advice regarding the phylogenetic analysis. The work was supported by the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (FORMAS), the European Commission (QLRT-99-1523), the Swedish Farmers Foundation for Agricultural Research and the Swedish Seed and Oil Seed Growers Association.

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Author notes

  1. Qiong Hu

    Present address: Institute of Oil Crops, Chinese Academy of Agricultural Sciences, 430062, Wuhan, People’s Republic of China

Affiliations

  1. Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, (SLU), P.O. Box 7080, 750 07, Uppsala, Sweden

    Jan Fahleson & Christina Dixelius

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  1. Jan Fahleson
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  2. Qiong Hu
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  3. Christina Dixelius
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Correspondence to Jan Fahleson.

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Fahleson, J., Hu, Q. & Dixelius, C. Phylogenetic analysis of Verticillium species based on nuclear and mitochondrial sequences. Arch Microbiol 181, 435–442 (2004). https://doi.org/10.1007/s00203-004-0677-1

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  • DOI: https://doi.org/10.1007/s00203-004-0677-1

Keywords

  • Mitochondrial and nuclear sequences
  • Partition-homogeneity test
  • Phylogeny
  • Verticillium species