Abstract
As has been previously shown, Saccharomyces cerevisiae grown in 2% or 0.025% glucose uses this carbohydrate by the fermentative or oxidative pathways, respectively. Depending on the glucose concentration in the medium, the effect of the addition of H2O2 on the level of ATP and on glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity differed. In the presence of 2% glucose, ATP and GAPDH decreased sharply during the first few minutes of treatment, whereas in the presence of 0.025% glucose, GAPDH activity decreased similarly, but the ATP level remained practically unchanged. The addition of 3 mM glutathione to the culture media prevented the depletion of ATP levels and GAPDH activity in the presence of H2O2. Catalase and superoxide dismutase activities did not vary significantly when yeast cells were grown either in 2% or in 0.025% glucose.
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Acknowledgements
This investigation was supported by grants from Dirección General de Investigación Científica y Técnica (BMC-2002-00866), Comunidad de Madrid (08/0021.1/2001), and Instituto de Salud Carlos III, RCMN (C03/08), Madrid, Spain. We thank Anabel de Diego and Cristina Almansa for their excellent technical assistance, Dr. Claudio F. Heredia and Dr. Eduardo Silles for helpful discussions, and Dr. Miguel Manzanares for critical reading of the manuscript. H.O. was supported by a fellowship from the Fundação para a Ciência e a Tecnología (SFRH/BD/1477/2000).
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Osório, H., Moradas-Ferreira, P., Günther Sillero, M.A. et al. In Saccharomyces cerevisiae, the effect of H2O2 on ATP, but not on glyceraldehyde-3-phosphate dehydrogenase, depends on the glucose concentration. Arch Microbiol 181, 231–236 (2004). https://doi.org/10.1007/s00203-004-0648-6
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DOI: https://doi.org/10.1007/s00203-004-0648-6