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Utilization of aminoaromatic acids by a methanogenic enrichment culture and by a novel Citrobacter freundii strain

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Abstract

Following incubation of mesophilic methanogenic floccular sludge from a lab-scale upflow anaerobic sludge bed reactor used to treat cattle manure wastewater, a stable 5-aminosalicylate-degrading enrichment culture was obtained. Subsequently, a Citrobacter freundii strain, WA1, was isolated from the 5-aminosalicylate-degrading methanogenic consortium. The methanogenic enrichment culture degraded 5-aminosalicylate completely to CH4, CO2 and NH4 +, while C. freundii strain WA1 reduced 5-aminosalicylate with simultaneous deamination to 2-hydroxybenzyl alcohol during anaerobic growth with electron donors such as pyruvate, glucose or serine. When grown on pyruvate, C. freundii WA1 converted 3-aminobenzoate to benzyl alcohol and also reduced benzaldehyde to benzyl alcohol. Pyruvate was fermented to acetate, CO2, H2 and small amounts of lactate, succinate and formate. Less lactate (30%) was produced from pyruvate when C. freundii WA1 grew with 5-aminosalicylate as co-substrate.

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Acknowledgements

We thank S. Parshina and V. Sklyar for stimulating discussions. This research was supported by the graduate school WIMEK and by RFBR grant no. 01-04-49601.

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Authors and Affiliations

  1. Department of Microbiology, Moscow State University, 119899 , Moscow, Russia

    Olga Savelieva, Irina Kotova & Alexander Netrusov

  2. Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 , CT Wageningen, The Netherlands

    Wim Roelofsen & Alfons J. M. Stams

Authors
  1. Olga Savelieva
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  2. Irina Kotova
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  3. Wim Roelofsen
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  4. Alfons J. M. Stams
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  5. Alexander Netrusov
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Correspondence to Olga Savelieva.

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Savelieva, O., Kotova, I., Roelofsen, W. et al. Utilization of aminoaromatic acids by a methanogenic enrichment culture and by a novel Citrobacter freundii strain. Arch Microbiol 181, 163–170 (2004). https://doi.org/10.1007/s00203-003-0645-1

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  • DOI: https://doi.org/10.1007/s00203-003-0645-1

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