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Two-dimensional gel electrophoresis analysis of the abundance of virulent exoproteins of group A streptococcus caused by environmental changes

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Abstract

Group A streptococci regulate the expression of virulence factors in response to environmental change. In order to investigate this mechanism, the growth of group A streptococci and the abundance of virulent exoprotein production in culture supernatant were analyzed by two-dimensional gel electrophoresis (2-D electrophoresis) under several culture conditions. Judging from alterations in their growth, group A streptococci were affected by various environmental stresses. Under high O2 and low CO2 concentrations, streptococcal pyrogenic exotoxin B (SpeB) and streptococcal pyrogenic exotoxin F (SpeF) significantly decreased, and the streptococcal inhibitor of complement (Sic) increased . At 30 °C, increases in endo-β-N-acetylglucosaminidase (EndoS) and α-amylase were also detected, while at 41 °C EndoS became undetectable and SpeB and SpeF decreased. Sic, SpeF and mitogenic factor 3 (Mf3) decreased when cells were cultured in higher NaCl concentrations, and EndoS disappeared following culture of the cells in high glucose concentration. An increase in acid phosphatase and a decrease in several other proteins were detected when the cells were cultivated in high iron concentrations. These results suggest that group A streptococci have a versatile adaptation system that responds to several environmental stresses by altering the level of exoprotein production.

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Abbreviations

SpeB :

Streptococcal pyrogenic exotoxin B

SpeF :

Streptococcal pyrogenic exotoxin F

Sic :

Streptococcal inhibitor of complement

EndoS :

Endo-β-N-acetylglucosaminidase

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Acknowledgements

We thank Dr. Ferretti of Oklahoma University for providing strain SF370. This work was supported by a Grant-in-Aid for Scientific Research (11670261 and 12557027) from the Ministry of Education, Culture, Sports, Science and Technology, and a grant for research on emerging and reemerging infectious disease (H12 Sinkou-7) from the Ministry of Health and Welfare, Japan.

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Authors and Affiliations

  1. Department of Molecular Bacteriology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya , Japan

    Tadahiro Nakamura, Tadao Hasegawa, Keizo Torii & Michio Ohta

  2. Department of Medicine, Division of Respiratory Diseases, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan

    Tadahiro Nakamura, Yoshinori Hasegawa & Kaoru Shimokata

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  1. Tadahiro Nakamura
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  2. Tadao Hasegawa
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  3. Keizo Torii
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  4. Yoshinori Hasegawa
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  5. Kaoru Shimokata
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  6. Michio Ohta
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Correspondence to Tadao Hasegawa.

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Nakamura, T., Hasegawa, T., Torii, K. et al. Two-dimensional gel electrophoresis analysis of the abundance of virulent exoproteins of group A streptococcus caused by environmental changes. Arch Microbiol 181, 74–81 (2004). https://doi.org/10.1007/s00203-003-0632-6

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  • DOI: https://doi.org/10.1007/s00203-003-0632-6

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