Application of DEPArrayTM technology for the isolation of white blood cells from cell mixtures in chimerism analysis

Einsatz der DEPArrayTM-Technologie zur Isolierung von Leukozyten aus Mischungen im Rahmen von Chimärismusbestimmungen

Abstract

After allogeneic bone marrow or blood stem cell transplantation, STR profiling is often used for monitoring chimerisms. For the evaluation reference samples taken from the patient before the transplantation and/or taken from the donor are needed. In the case described here, no reference samples were available. Reporting the chimerism based on posttransplantation samples did not yield conclusive results; therefore, white blood cells and epithelial cells obtained from a buccal swab taken from the patient were separated using DEPArrayTM technology and STR profiling was carried out resulting in distinct reference profiles.

Zusammenfassung

Die Untersuchung von STR-Polymorphismen ist eine weit verbreitete Methode zur Bestimmung des chimären Status eines Patienten nach einer allogenen Knochenmark- oder Blutstammzelltransplantation. Für die Beurteilung werden Referenzproben vom Patienten vor Transplantation und/oder vom Spender benötigt. Diese lagen im hier geschilderten Fall nicht vor. Eine Chimärismusbestimmung an nach Transplantation entnommenen Proben brachte keine eindeutigen Ergebnisse. Erst mithilfe der DEPArrayTM-Technologie konnten aus einem Mundschleimhautabstrich des Patienten Leukozyten und Epithelzellen separiert, einzeln typisiert und somit eindeutige Referenzprofile erhalten werden.

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Acknowledgements

We thank the company Menarini Silicon Biosystems (Italy) for the provision of the DEPArrayTM system, as well as for the valuable support.

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Correspondence to PD Dr. K. Anslinger.

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K. Anslinger, B. Bayer and D. von Máriássy declare that they have no competing interests.

This article does not contain any studies with human participants or animals performed by any of the authors.

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Anslinger, K., Bayer, B. & von Máriássy, D. Application of DEPArrayTM technology for the isolation of white blood cells from cell mixtures in chimerism analysis. Rechtsmedizin 28, 134–137 (2018). https://doi.org/10.1007/s00194-017-0221-7

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Keywords

  • Allogeneic stem cell transplantation
  • Chimerism monitoring
  • STR profiling
  • Fluorescent labeling
  • DEPArrayTM

Schlüsselwörter

  • Allogene Stammzelltransplantation
  • Chimärismusbestimmung
  • STR-Typisierung
  • Fluoreszenzmarkierung
  • DEPArrayTM