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Type III Collagen RNA Level Expression in Pelvic Organ Prolapse: A Systematic Review and Meta-Analysis

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Abstract

Introduction and Hypothesis

Changes in the expression of type III collagen have been linked to women's predisposition to pelvic organ prolapse (POP); however, the findings of prior studies have been conflicting. This study was aimed at investigating whether changes in the type III collagen gene expression levels occur in POP development.

Methods

A systematic review and meta-analysis were conducted on research articles that evaluated type III collagen gene expression levels in patients with POP compared with those without the condition. The articles, published between January 2000 and February 2024, were obtained from PubMed, ScienceDirect, Semantic Scholar, and EBSCO databases. Data were analyzed using fixed-effect models, and the pooled standardized mean difference (SMD) was calculated. Cochrane’s Review Manager 5.4 was used for the analysis. The aggregated SMD with 95% confidence interval (CI) regarding type III collagen gene expression levels relative to POP development was the main outcome measure. Results with p < 0.05 were considered statistically significant.

Results

Six studies were included in our analysis, comprising 229 POP cases and 139 non-POP cases. Our meta-analysis indicated that patients with POP had higher type III collagen gene expression levels than those without POP (SMD = 0.32; 95% CI: 0.07 to 0.56; p = 0.01).

Conclusion

The results of this study provide evidence that a higher type III collagen gene expression levels is significantly associated with POP.

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Data Availability

The corresponding author can provide the data findings upon reasonable request.

Abbreviations

Col3A1:

Collagen type III alpha 1 chain

MD:

Mean difference

mRNA:

Messenger ribonucleic acid

NOS:

Newcastle Ottawa Quality Assessment Scale

POP:

Pelvic organ prolapse

POP-Q:

Pelvic organ prolapse quantification

PRISMA:

The Preferred Reporting Items for Systematic Reviews and Meta-Analyses

SMD:

Standardized mean difference

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Acknowledgements

The authors would like to express their gratitude to the native speaking-staff of Klinik Bahasa FKKMK Universitas Gadjah Mada for proofreading and language assistance.

Funding

The authors received funding from Universitas Gadjah Mada for the Final Project Recognition Grant, with reference number 5075/UNI.P.II/Dit-Lit/PT.01.01/2023.

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Authors and Affiliations

  1. Doctoral Program, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia

    Akbar Novan Dwi Saputra

  2. Department of Physiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia

    Dicky Moch Rizal & Nandia Septiyorini

  3. Department of Obstetrics and Gynecology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia

    Akbar Novan Dwi Saputra & Muhammad Nurhadi Rahman

Authors
  1. Akbar Novan Dwi Saputra
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Contributions

A.N.D.S. participated in designing the study, interpreting the data, and writing and reviewing the manuscript; D.M.R. was involved in designing the study and reviewing the manuscript; N.S. contributed to interpreting the data, and writing and editing the manuscript; M.N.R. played a role in contributing to manuscript writing and reviewing.

Corresponding author

Correspondence to Dicky Moch Rizal.

Ethics declarations

As this study is a systematic review and meta-analysis of previously published data, it did not require ethics approval or patient consent. No primary data collection or human subjects research was conducted for this study.

Conflicts of Interest

The authors have declared that no conflicts of interest exist.

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Saputra, A.N.D., Rizal, D.M., Septiyorini, N. et al. Type III Collagen RNA Level Expression in Pelvic Organ Prolapse: A Systematic Review and Meta-Analysis. Int Urogynecol J (2024). https://doi.org/10.1007/s00192-024-05953-7

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