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An in vitro pilot study investigating placenta-derived mesenchymal stem cell coating on polypropylene mesh materials

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Abstract

Introduction and hypothesis

Polypropylene meshes (PM) used in pelvic organ prolapse surgery are being withdrawn from the market. Although concerns about the usage of PMs in stress incontinence surgery have been raised, it is still one of the best methods of curing stress urinary incontinence. With advancements in stem cell-based therapies, especially mesenchymal stem cells (MSCs), it is believed that coating the synthetic meshes with MSCs may minimize excessive tissue reactions ultimately leading to clinical problems such as pain, erosion or extrusion of the implanted material. In our study we tried to show the possibility of coating the PM with placenta-derived MSCs.

Methods

Mesenchymal stem cells obtained from six placentas were isolated, cultured, and identified. MSCs were then soaked in either fibronectin or collagen prior to co-culturing with strips of PMs. One group is used as a control, and hence was not pretreated before co-culturing. Specimens were fixed and stained with both Gram and hematoxylin and eosin and marked with Vybran Dil and DAPI. All preparations were examined under a light microscope. The IMAGEJ program was utilized to determine the surface area of meshes coated with MSCs.

Results

We clearly showed that PMs can be coated successfully with placenta-derived MSCs. The percentage of the coated area is significantly increased when meshes were pretreated with fibronectin or collagen (p<0.0001).

Conclusions

Placenta-derived MSCs can successfully coat PMs. The immunomodulatory properties of MSCs, which may be of great advantage in preventing the side effects of meshes, should be tested by in vivo and hopefully human studies before clinical applications.

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Acknowledgements

We would like to extend our gratitude to Biologist Hatice Irday, Biologist Sibel Erkoç and, Gülcin Daglioglu, MD, from the Central Laboratory of Çukurova University, Adana, Turkey, for their valuable support in flow cytometry analyses.

Funding

This study was funded by Alanya Alaaddin Keykubat University Scientific Research and Project Board (2021-04-03-MAP01).

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Authors and Affiliations

  1. Faculty of Medicine, Department of Obstetrics and Gynecology, Alanya Alaaddin Keykubat University, Payallar Mh. Atatürk Cd. Yasemin Villaları, No:111/L-4, Alanya, Antalya, Türkiye

    Erdogan Aslan & Emre Köle

  2. Faculty of Medicine, Department of Pharmacology, Alanya Alaaddin Keykubat University, Alanya, Antalya, Türkiye

    Erkan Maytalman, Dilara Nemutlu Samur & Özlem Ceren Günizi

Authors
  1. Erdogan Aslan
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  2. Erkan Maytalman
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  3. Dilara Nemutlu Samur
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  4. Emre Köle
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  5. Özlem Ceren Günizi
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Contributions

E. Aslan: project development, manuscript writing; E. Maytalman: data collection, laboratory experiments; D. Nemutlu Samur: data collection, laboratory experiments; E. Köle: data collection, manuscript writing; Ö.C. Günizi: data collection, laboratory experiments.

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Correspondence to Erdogan Aslan.

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Aslan, E., Maytalman, E., Nemutlu Samur, D. et al. An in vitro pilot study investigating placenta-derived mesenchymal stem cell coating on polypropylene mesh materials. Int Urogynecol J 35, 553–559 (2024). https://doi.org/10.1007/s00192-023-05687-y

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  • DOI: https://doi.org/10.1007/s00192-023-05687-y

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