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How antepartum and postpartum acute urinary retention affects the function and structure of the rat bladder

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Abstract

Introduction and hypothesis

To examine the effect of acute urinary retention (AUR) on the urodynamic function and molecular structure of rat bladders in pregnancy and after parturition.

Methods

Forty-eight nulliparous rats were distributed into AUR and non-AUR groups. AUR was induced by clamping the distal urethra of each rat after infusing 3 ml of saline for 60 min. Plasma progesterone levels and cystometric data were evaluated on the 14th day of gestation, 3 and 10 days postpartum, and in virgin rats. The immunoreactivity of caveolins and nerve growth factor (NGF) was analyzed. The number of caveolae in bladder muscle cells was evaluated by electron microscopy.

Results

Progesterone levels significantly increased during pregnancy and 3 days postpartum. In cystometric results, the AUR group has significantly shorter intercontraction interval, lower void volume and greater residual volume compared with the non-AUR. AUR rats exhibited higher NGF immunoreactivity, lower caveolin-1 immunoreactivity, and less caveolae in the bladder compared with the non-AUR. The caveolin-1 and NGF immunoreactivity and the number of caveolae in the bladder decreased during pregnancy and 3 days postpartum compared with virgin rats. By using Pearson correlations, we found significant correlations between urodynamic variables (residual volumes and intercontraction intervals) and the expressions of caveolin-1, caveolae and NGF in the AUR rats on the 14th day of gestation and 3 days postpartum.

Conclusions

Bladder dysfunction in pregnancy and immediately postpartum in a rat model caused by AUR is associated with the plasma progesterone level change and the expressions of caveolin, caveolae, and NGF in bladder muscle cells.

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Abbreviations

AUR:

Acute urinary retention

BOO:

Bladder outlet obstruction

OD:

Optical density

NGF:

Nerve growth factor

PBS:

Phosphate buffered saline

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Acknowledgements

This work was supported by the Medical Research Project, Chang Gung Memorial Hospital (grant numbers CMRPG300021-2 and BMRP412). The authors would like to thank Dr. Yao-Lung Chang for his assistance in accomplishing statistical analyses.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Linkou Medical Center, 5, Fu-Shin Street, Kweishan, Taoyuan, Taiwan, Republic of China, 333

    Ching-Chung Liang, Yi-Hao Lin & Shuenn-Dhy Chang

  2. Department of Obstetrics and Gynecology, College of Medicine, Chang Gung University, Taoyuan, Taiwan

    Ching-Chung Liang, Yi-Hao Lin, Tse-Ching Chen & Shuenn-Dhy Chang

  3. Department of Pathology, Chang Gung Memorial Hospital, Linkou Medical Center, Taoyuan, Taiwan

    Tse-Ching Chen

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  1. Ching-Chung Liang
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  2. Yi-Hao Lin
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Correspondence to Ching-Chung Liang.

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Liang, CC., Lin, YH., Chen, TC. et al. How antepartum and postpartum acute urinary retention affects the function and structure of the rat bladder. Int Urogynecol J 25, 1105–1113 (2014). https://doi.org/10.1007/s00192-013-2320-7

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  • DOI: https://doi.org/10.1007/s00192-013-2320-7

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