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Estrogen inhibits vaginal tropoelastin and TGF-β1 production

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Abstract

Introduction and hypothesis

The aim of this study was to quantify the effects of estrogen on vaginal smooth muscle cell (SMC) tropoelastin and transforming growth factor (TGF)-β1 production.

Methods

Primary SMC were incubated with estradiol, and cell proliferation was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay at 48 h. Supernatants were collected and tropoelastin and TGF-β1 levels measured.

Results

SMC proliferation was significantly increased by estradiol [relative cell number, mean ± standard error (SE), estradiol 0.1 μM 116 ± 19 % of control (P = NS), 1 μM 127 ± 13 % of control (P < 0.05), 10 μM 153 ± 26 % of control, (P < 0.05)]. Tropoelastin production was significantly decreased by estrogen [mean ± SE, estradiol 0.1 μM 78 ± 2 % of control (P < 0.05), 1 μM 76 ± 4 % of control (P < 0.05), 10 μM 67 ± 3 % of control, (P < 0.05)]. In addition, TGF-β1 production was significantly decreased [mean ± SE, estradiol 0.1 μM 96 ± 4 % of control (P = NS), 1 μM 84 ± 6 % of control (P < 0.05), 10 μM 70 ± 6 % of control, (P < 0.05)].

Conclusion

Estrogen increases vaginal SMC proliferation and inhibits tropoelastin and TGF-β1 production.

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References

  1. Milson I, Altman D, Lapitan MC et al (2009) Epidemiology of urinary and faecal incontinence and pelvic organ prolapse. In: Abrams P et al (eds) Incontinence, 4th edn. Health Publication, UK pp 35–112

  2. Chen B, Yeh J (2011) Alterations in Connective Tissue Metabolism in Stress Incontinence and Prolapse. J Urology 186:1768–1772

    Article  CAS  Google Scholar 

  3. Goepel C, Thomssen C (2006) Changes in the extracellular matrix in periurethral tissue of women with stress urinary incontinence. Acta Histocem 108(6):441–5

    Article  Google Scholar 

  4. Li B, Zhang QF, Lin XY, Chen LL, Ouyang L, Zhang SL (2009) Expression and significance of elastin and fibulin-5 in anterior vaginal tissue of women with pelvic organ prolapse .Zhonghua Fu Chan KeZaZhi. 44(7):514–7

  5. Cody JD, Richardson K, Moehrer B, Hextall A, Glazener, CAM (2009) Oestrogen therapy for urinary incontinence in post-menopausal women. Cochrane Database Syst Rev. 4:CD001405

  6. Steinauer JE, Waetjen LE, Vittinghoff E, Subak LL, Hulley SB, Grady D, Lin F, Brown JS (2005) Postmenopausal Hormone Therapy: Does It Cause Incontinence? Obstet Gynecol 106(5 Pt 1):940–945

    Article  PubMed  CAS  Google Scholar 

  7. Hendrix SL, Cochrane BB, Nygaard IE, Handa VL, Barnabei VM, Iglesia C, Aragaki A, Naughton MJ, Wallace RB, McNeeley SG (2005) Effects of estrogen with and without progestin on urinary incontinence. JAMA 293(8):935–48

    Article  PubMed  CAS  Google Scholar 

  8. Goldstein SR, Nanvati N (2002) Adverse events that are associated with the selective estrogen receptor modulator levormeloxifene in an aborted phase III osteoporosis treatment study. Am J Obstet Gynecol 187:521

    Article  PubMed  CAS  Google Scholar 

  9. Skrumsager BK, Kiehr B, Pedersen PC, Gerrits M, Watson N, Bjarnason K (2002) Levormeloxifene: safety, pharmacodynamics and pharmacokinetics in healthy postmenopausal women following single and multiple doses of a new selective oestrogen receptor modulator. Br J Clin Pharmacol 53(3):284–95

    Article  PubMed  CAS  Google Scholar 

  10. Alexandersen P, Toussaint A, Christiansen C, Devogelaer JP, Roux C, Fechtenbaum J, Gennari C, Reginster JY (2001) Ipriflavone in the treatment of postmenopausal osteoporosis: a randomized controlled trial. Ipriflavone Multicenter European Fracture Study. JAMA 285(11):1482–8

    Article  PubMed  CAS  Google Scholar 

  11. Takacs P, Yavagal S, Zhang Y, Candiotti K, Medina CA (2011) Levormeloxifene inhibits vaginal tropoelastin and transforming growth factor beta 1 production. J Smooth Muscle Res 47(1):11–9

    Article  PubMed  Google Scholar 

  12. Takacs P, Zhang Y, Yavagal S, Candiotti K, Chakhtoura N, Medina CA (2011) TGF-beta 1 is a potential regulator of vaginal tropoelastin production. Int Urogynecol J [Epub ahead of print]

  13. Takacs P, Zhang Y, Jaramillo S, Bardawil T, Candiotti K, Medina CA (2010) The effects of estrogen, progesterone and polypropylene mesh on vaginal smooth muscle cell proliferation. J Smooth Muscle Res 46:9–15

    Article  PubMed  Google Scholar 

  14. Northington GM, de Vries HF, Bogner HR (2012) Self-reported estrogen use and newly incident urinary incontinence among postmenopausal community-dwelling women. Menopause 19(3) [Epub ahead of print]

  15. Kahari VM, Olsen DR, Rhudy RW, Carrillo P, Chen YQ, Uitto J (1992) Transforming growth factor-beta up-regulates elastin gene expression in human skin fibroblasts. Evidence for post-transcriptional modulation. Lab Invest 66:580–588

    PubMed  CAS  Google Scholar 

  16. Kuang PP, Zhang XH, Rich CB, Foster JA, Subramanian M, Goldstein RH (2007) Activation of elastin transcription by transforming growth factor-beta in human lung fibroblasts. Am J Physiol Lung Cell Mol Physiol 292:L944–L952

    Article  PubMed  CAS  Google Scholar 

  17. Katchman SD, Hsu-Wong S, Ledo I, Wu M, Uitto J (1994) Transforming growth factor-beta up-regulates human elastin promoter activity in transgenic mice. Biochem Biophys Res Commun 203:485–490

    Article  PubMed  CAS  Google Scholar 

  18. Selzman CH, Gaynor JS, Turner AS, Whitehill TA, Horwitz LD, Harken AH (1998) Estrogen replacement inhibits intimal hyperplasia and the accumulation and effects of transforming growth factor beta1. J Surg Res 80(2):380–5

    Article  PubMed  CAS  Google Scholar 

  19. Ito I, Hanyu A, Wayama M, Goto N, Katsuno Y, Kawasaki S, Nakajima Y, Kajiro M, Komatsu Y, Fujimura A, Hirota R, Murayama A, Kimura K, Imamura T, Yanagisawa J (2010) Estrogen inhibits transforming growth factor beta signaling by promoting Smad2/3 degradation. J Biol Chem 285(19):147–55

    Article  Google Scholar 

  20. Takahashi T, Eitzman B, Bossert NL, Walmer D, Sparrow K, Flanders KC, McLachlan J, Nelson KG (1994) Transforming growth factors beta 1, beta 2, and beta 3 messenger RNA and protein expression in mouse uterus and vagina during estrogen-induced growth: a comparison to other estrogen-regulated genes. Cell Growth Differ 5(9):919–35

    PubMed  CAS  Google Scholar 

  21. Wen Y, Polan ML, Chen B (2006) Do extracellular matrix protein expressions change with cyclic reproductive hormones in pelvic connective tissue from women with stress urinary incontinence? Hum Reprod 21(5):1266–73

    Article  PubMed  CAS  Google Scholar 

  22. Lin G, Ning H, Wang G, Banie L, Lue TF, Lin CS (2010) Effects of birth trauma and estrogen on urethral elastic fibers and elastin expression. Urology 76(4):1018.e8–13

    Article  Google Scholar 

  23. Chen B, Wen Y, Yu X, Polan ML (2005) Elastin metabolism in pelvic tissues: is it modulated by reproductive hormones? Am J Obstet Gynecol 192(5):1605–15

    Article  PubMed  CAS  Google Scholar 

  24. Liu YM, Choy KW, Lui WT, Pang MW, Wong YF, Yip SK (2006) 17beta-estradiol suppresses proliferation of fibroblasts derived from cardinal ligaments in patients with or without pelvic organ prolapse. Hum Reprod 21:303–308

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology (D-50), University of Miami, Miller School of Medicine, P.O. Box 016960, Miami, FL, 33010, USA

    Nahida Chakhtoura, Carlos A. Medina & Peter Takacs

  2. Department of Anesthesiology, University of Miami, Miller School of Medicine, P.O. Box 016960, Miami, FL, 33010, USA

    Yanping Zhang & Keith Candiotti

Authors
  1. Nahida Chakhtoura
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  2. Yanping Zhang
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  3. Keith Candiotti
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  4. Carlos A. Medina
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  5. Peter Takacs
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Corresponding author

Correspondence to Peter Takacs.

Additional information

Takacs: project development, data analysis, manuscript writing; Zhang: data collection and analysis; Candiotti: project development, manuscript writing; Chakhtoura: project development, manuscript writing; Medina: project development, manuscript writing.

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Chakhtoura, N., Zhang, Y., Candiotti, K. et al. Estrogen inhibits vaginal tropoelastin and TGF-β1 production. Int Urogynecol J 23, 1791–1795 (2012). https://doi.org/10.1007/s00192-012-1828-6

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  • DOI: https://doi.org/10.1007/s00192-012-1828-6

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