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The impact of simulated birth trauma and ovariectomy on the gene expression of detrusor muscarinic receptors in female rats

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Abstract

Introduction and hypothesis

This study aims to investigate the effects of simulated birth trauma and ovariectomy on detrusor muscarinic receptors (M2 and M3), urethral neuronal nitric oxide synthase (nNOS), and estrogen receptor β (ERβ).

Methods

Forty primiparous rats were equally divided into five groups: group A—delivery, group B—delivery plus ovariectomy, group C—delivery plus balloon dilatation for 2 h, group D—delivery plus balloon dilatation for 4 h, and group E—delivery plus balloon dilatation for 2 h plus ovariectomy. The gene expression of M2, M3, nNOS, and ERβ were assessed by reverse transcription polymerase chain reaction.

Results

Significant decreases in mRNA expression of M2 receptors and nNOS (P < 0.05), and a significant increase in M3 mRNA expression (P < 0.05) were observed in groups D and E when compared with group A.

Conclusions

Ovariectomy following birth trauma may synergistically impact the function of urinary tract, this being possibly related to the modification of the gene expression of muscarinic receptors.

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Abbreviations

LUTS:

lower urinary tract symptoms

OAB:

overactive bladder

NO:

nitric oxide

NOS:

nitric oxide synthase

nNOS:

neuronal nitric oxide synthase

ER α:

estrogen receptor α

ER β:

estrogen receptor β

RT-PCR:

reverse transcription polymerase chain reaction

cDNA:

complementary DNA

References

  1. van Brummen HJ, Bruinse HW, van de Pol G, Heintz AP, van der Vaart CH (2007) The effect of vaginal and cesarean delivery on lower urinary tract symptoms: what makes the difference? Int Urogynecol J Pelvic Floor Dysfunct 18:133–139

    PubMed  Google Scholar 

  2. Chen GD, Lin TL, Hu SW, Chen YC, Lin LY (2003) Prevalence and correlation of urinary incontinence and overactive bladder in Taiwanese women. Neurourol Urodyn 22:109–117

    Article  PubMed  Google Scholar 

  3. Yamaguchi O, Shishido K, Tamura K, Ogawa T, Fujimura T, Ohtsuka M (1996) Evaluation of mRNAs encoding muscarinic receptor subtypes in human detrusor muscle. J Urol 156:1208–1213

    Article  CAS  PubMed  Google Scholar 

  4. Eglen RM, Hegde SS, Watson N (1996) Muscarinic receptor subtypes and smooth muscle function. Pharmacol Rev 48:531–565

    CAS  PubMed  Google Scholar 

  5. Andersson KE, Persson K (1994) Nitric oxide synthase and nitric oxide-mediated effects in lower urinary tract smooth muscles. World J Urol 12:274–280

    CAS  PubMed  Google Scholar 

  6. Mamas MA, Reynard JM, Brading AF (2003) Nitric oxide and the lower urinary tract: current concepts, future prospects. Urology 61:1079–1085

    Article  PubMed  Google Scholar 

  7. Vizzard MA, Erdman SL, Förstermann U, de Groat WC (1994) Differential distribution of nitric oxide synthase in neural pathways to the urogenital organs (urethra, penis, urinary bladder) of the rat. Brain Res 646:279–291

    Article  CAS  PubMed  Google Scholar 

  8. Peterson CM (2000) Estrogen and progesterone receptors: an overview from the year 2000. J Soc Gynecol Investig 7(1 Suppl):S3–S7

    Article  CAS  PubMed  Google Scholar 

  9. Chen GD, Oliver RH, Leung BS, Lin LY, Yeh J (1999) Estrogen receptor alpha and beta expression in the vaginal walls and uterosacral ligaments of premenopausal and postmenopausal women. Fertil Steril 71:1099–1102

    Article  CAS  PubMed  Google Scholar 

  10. Wu R, Yin J, Yao K, Haefliger I (2007) Inhibition by brimonidine of forskolin-induced nitric oxide synthase expression in human ciliary bodies in vitro. Mol Vis 13:493–496

    CAS  PubMed  Google Scholar 

  11. Mimata H, Nomura Y, Emoto A, Latifpour J, Wheeler M, Weiss RM (1997) Muscarinic receptor subtypes and receptor-coupled phosphatidylinositol hydrolysis in rat bladder smooth muscle. Int J Urol 4:591–596

    Article  CAS  PubMed  Google Scholar 

  12. Agras K, Willingham E, Shiroyanagi Y, Minasi P, Baskin LS (2007) Estrogen receptor-alpha and beta are differentially distributed, expressed and activated in the fetal genital tubercle. J Urol 177:2386–2392

    Article  CAS  PubMed  Google Scholar 

  13. Andersson KE (1999) Advances in the pharmacological control of the bladder. Exp Physiol 84:195–213

    CAS  PubMed  Google Scholar 

  14. Nilvebrant L, Andersson KE, Mattiasson A (1985) Characterization of the muscarinic cholinoceptors in the human detrusor. J Urol 134:418–423

    CAS  PubMed  Google Scholar 

  15. Tong YC, Hung YC, Lin SN, Cheng JT (1997) Pharmacological characterization of the muscarinic receptor subtypes responsible for the contractile response in the rat urinary bladder. J Auton Pharmacol 17:21–25

    Article  PubMed  Google Scholar 

  16. Blakeman PJ, Hilton P, Bulmer JN (2000) Oestrogen and progesterone receptor expression in the female lower urinary tract, with reference to oestrogen status. BJU Int 86:32–38

    Article  CAS  PubMed  Google Scholar 

  17. Sievert KD, Emre Bakircioglu M, Tsai T, Dahms SE, Nunes L, Lue TF (2001) The effect of simulated birth trauma and/or ovariectomy on rodent continence mechanism. Part I: functional and structural change. J Urol 166:311–317

    Article  CAS  PubMed  Google Scholar 

  18. Liang W, Afshar K, Stothers L, Laher I (2002) The influence of ovariectomy and estrogen replacement on voiding patterns and detrusor muscarinic receptor affinity in the rat. Life Sci 71:351–362

    Article  CAS  PubMed  Google Scholar 

  19. Lin AS, Carrier S, Morgan DM, Lue TF (1998) Effect of simulated birth trauma on the urinary continence mechanism in the rat. Urology 52:143–151

    Article  CAS  PubMed  Google Scholar 

  20. Long CY, Hsu CS, Shao PL, Liu CM, Su JH, Tsai EM (2009) Effect of ovariectomy on the gene expression of detrusor muscarinic receptors in female rats. Fertil Steril 92:1147–1149

    Article  CAS  PubMed  Google Scholar 

  21. Shapiro E (1986) Effect of estrogens on the weight and muscarinic cholinergic receptor density of the rabbit bladder and urethra. J Urol 135:1084–1087

    CAS  PubMed  Google Scholar 

  22. Long CY, Liu CM, Hsu SC, Wu CH, Wang CL, Tsai EM (2006) A randomized comparative study of the effects of oral and topical estrogen therapy on the vaginal vascularization and sexual function in hysterectomized postmenopausal women. Menopause 13:737–743

    Article  PubMed  Google Scholar 

  23. Long CY, Liu CM, Hsu SC, Chen YH, Wu CH, Tsai EM (2006) A randomized comparative study of the effects of oral and topical estrogen therapy on the lower urinary tract of hysterectomized postmenopausal women. Fertil Steril 85:155–160

    Article  CAS  PubMed  Google Scholar 

  24. Carley ME, Cliby WA, Spelsberg TC (2002) P2X(3) receptor subunit messenger RNA expression in the female mouse bladder after oophorectomy with or without estrogen replacement. Am J Obstet Gynecol 187:103–106

    Article  CAS  PubMed  Google Scholar 

  25. Carley ME, Rickard DJ, Gebhart JB, Webb MJ, Podratz KC, Spelsberg TC (2003) Distribution of estrogen receptors alpha and beta mRNA in mouse urogenital tissues and their expression after oophorectomy and estrogen replacement. Int Urogynecol J Pelvic Floor Dysfunct 14:141–145

    Article  PubMed  Google Scholar 

  26. Viktrup L, Lose G (2008) Incidence and remission of lower urinary tract symptoms during 12 years after the first delivery: a cohort study. J Urol 180:992–997

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

The authors appreciate that this study was supported by the grants from the National Science Council and Education of Ministry of the Republic of China (NSC 93-2314-B-037-063; KMU-EM-1.3.c, KMU-EM-1.3.d).

Conflicts of interest

None

Author information

Authors and Affiliations

  1. Graduate Institute of Medicine, Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

    Cheng-Yu Long, Cheng-Min Liu & Eing-Mei Tsai

  2. Department of Obstetrics and Gynecology, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan

    Cheng-Yu Long, Yung-Hung Chen & Chiu-Lin Wang

  3. Department of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan

    Chin-Hu Wu & Eing-Mei Tsai

  4. Department of Obstetrics and Gynecology, Kaohsiung Municipal Hsiao-Kang Hospital, 482 Shan-Ming Road, Hsiao-Kang Dist. 812, Kaohsiung, Taiwan

    Eing-Mei Tsai

Authors
  1. Cheng-Yu Long
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  2. Chin-Hu Wu
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  3. Cheng-Min Liu
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  4. Yung-Hung Chen
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  5. Chiu-Lin Wang
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  6. Eing-Mei Tsai
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Corresponding author

Correspondence to Eing-Mei Tsai.

Additional information

Cheng-Yu Long and Chin-Hu Wu contributed equally to this work.

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Long, CY., Wu, CH., Liu, CM. et al. The impact of simulated birth trauma and ovariectomy on the gene expression of detrusor muscarinic receptors in female rats. Int Urogynecol J 21, 1163–1168 (2010). https://doi.org/10.1007/s00192-010-1159-4

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  • DOI: https://doi.org/10.1007/s00192-010-1159-4

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